{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T15:06:07Z","timestamp":1782140767459,"version":"3.54.5"},"reference-count":24,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Physiol"],"abstract":"<i>Background:<\/i> Hypomagnesemia with secondary hypocalcemia is due to disturbed renal and intestinal magnesium (Mg<sup>2+<\/sup>) (re)absorption. The underlying defect is a mutation in the transient receptor potential melastatin type 6 (TRPM6), a Mg<sup>2+<\/sup>-permeable ion channel expressed in the kidney and intestine. Our aim was to characterize homozygous (\u2013\/\u2013) and heterozygous (+\/\u2013) TRPM6 knockout mice with respect to Mg<sup>2+<\/sup> homeostasis. <i>Methods:<\/i> TRPM6<sup>+\/\u2013<\/sup> mice were bred on a normal (0.19% wt\/wt Mg<sup>2+<\/sup>) and high (0.48% wt\/wt Mg<sup>2+<\/sup>) Mg<sup>2+<\/sup> diet. In the offspring, 24-hour urinary Mg<sup>2+<\/sup> and calcium excretion as well as serum concentrations of both were determined. TRPM6 mRNA expression in the kidney and colon was measured. <i>Results:<\/i> On the regular diet, 30% of the offspring were TRPM6 wild-type (<sup>+\/+<\/sup>), 70% were TRPM6<sup>+\/\u2013<\/sup>, and none were TRPM6<sup>\u2013\/\u2013<\/sup>. The genotypic distribution of the litters remained the same on the 0.48% Mg<sup>2+<\/sup> diet. In TRPM6<sup>+\/\u2013<\/sup> mice on both diets, serum Mg<sup>2+<\/sup> levels were significantly lower, and renal and intestinal TRPM6 mRNA expression was reduced. Urinary Mg<sup>2+<\/sup> excretion was unaffected. <i>Conclusions:<\/i> Homozygous TRPM6 deletion is embryonic lethal in mice. Heterozygous deletion of TRPM6 results in a mild hypomagnesemia. The Mg<sup>2+<\/sup>-enriched diet could not compensate for either embryonic lethality or hypomagnesemia caused by TRPM6 deficiency.<\/jats:p>","DOI":"10.1159\/000320580","type":"journal-article","created":{"date-parts":[[2010,9,3]],"date-time":"2010-09-03T14:38:27Z","timestamp":1283524707000},"page":"p11-p19","source":"Crossref","is-referenced-by-count":65,"title":["Transient Receptor Potential Melastatin 6 Knockout Mice Are Lethal whereas Heterozygous Deletion Results in Mild Hypomagnesemia"],"prefix":"10.1159","volume":"117","author":[{"given":"Titia E.","family":"Woudenberg-Vrenken","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjaree","family":"Sukinta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemiete W.","family":"van der Kemp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 J.M.","family":"Bindels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost G.J.","family":"Hoenderop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2010,9,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01868686"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI115317"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007%2FBF00492330"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1136%2Fgut.20.11.1028"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fng901"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.177.4049.606"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1136%2Fadc.78.2.127"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2004110989"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038%2Fng889"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M311201200"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2F35079092"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0305252101"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M611117200"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00431-008-0767-1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2005070700"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI31680"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2008030327"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI36948"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1058519"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0092-8674%2803%2900556-7"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cub.2005.02.050"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1163493"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1093%2Fhmg%2Fddp392"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.8016642"}],"container-title":["Nephron Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/320580","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/320580","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:52:19Z","timestamp":1745437939000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000320580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,1]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2011,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000320580","archive":["Portico"],"relation":{},"ISSN":["1660-2137"],"issn-type":[{"value":"1660-2137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,1]]}}}