{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:16:50Z","timestamp":1790824610615,"version":"4.1.0"},"reference-count":30,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>With only 688 protein-coding genes, &lt;i&gt;Mycoplasma pneumoniae&lt;\/i&gt; is one of the smallest self-replicating organisms. These bacteria use glycolysis as the major pathway for ATP production by substrate-level phosphorylation, suggesting that this pathway must be optimized to high efficiency. In this study, we have investigated the interactions between glycolytic enzymes using the bacterial adenylate cyclase-based two-hybrid system. We demonstrate that most of the glycolytic enzymes perform self-interactions, suggesting that they form dimers or other oligomeric forms. In addition, enolase was identified as the central glycolytic enzyme of &lt;i&gt;M. pneumoniae&lt;\/i&gt; due to its ability to directly interact with all other glycolytic enzymes. Our results support the idea of the formation of a glycolytic complex in &lt;i&gt;M. pneumoniae&lt;\/i&gt; and we suggest that the formation of this complex might ensure higher fluxes through the glycolytic pathway than would be possible with isolated non-interacting enzymes.<\/jats:p>","DOI":"10.1159\/000321499","type":"journal-article","created":{"date-parts":[[2010,10,6]],"date-time":"2010-10-06T07:39:47Z","timestamp":1286350787000},"page":"134-139","source":"Crossref","is-referenced-by-count":22,"title":["Interactions between Glycolytic Enzymes of &lt;i&gt;Mycoplasma pneumoniae&lt;\/i&gt;"],"prefix":"10.1159","volume":"19","author":[{"given":"Pavel","family":"Dutow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian R.","family":"Schmidl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meike","family":"Ridderbusch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"St\u00fclke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2010,10,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1186%2F1752-0509-2-27"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1152241"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1574-6976.2008.00129.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1128%2FMMBR.69.4.608-634.2005"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0409741102"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.082112499"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.micro.61.080706.093440"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2958.2008.06210.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fpath.1700950229"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1074%2Fmcp.M800546-MCP200"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2FS0968-0004%2898%2901274-2"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10096-008-0591-6"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0959-440X%2800%2900154-8"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jmb.2007.05.098"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1128%2FJB.186.23.7936-7943.2004"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159%2F000096470"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1128%2FAEM.71.7.4097-4100.2005"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F24.22.4420"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M700198200"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.95.10.5752"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.tibs.2005.01.005"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1176343"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2958.2001.02523.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1432-1033.1976.tb10421.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1089%2F15362310260256909"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-4632.2009.04154.x"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1074%2Fmcp.M900267-MCP200"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1128%2FCMR.17.4.697-728.2004"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016%2F0006-3002%2858%2990533-X"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1177263"}],"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/321499","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/321499","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:00:53Z","timestamp":1745434853000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000321499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":30,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2010,12,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000321499","archive":["Portico"],"relation":{},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"value":"2673-1665","type":"print"},{"value":"2673-1673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}