{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:36:44Z","timestamp":1745469404313,"version":"3.40.4"},"reference-count":24,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Human papillomavirus (HPV) testing coupled with appropriate clinical management is associated with a significant decline in the rate of advanced cervical cancer and associated death. &lt;i&gt;Methods:&lt;\/i&gt; In this present study, we evaluated the performance of 2 new HPV genotyping methods, matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and surface plasmon resonance (SPR) in 30 kinds of HPV control materials and in 129 cases of cervical smears including 79 HPV-positive samples screened from 1,600 abnormal clinical samples and 50 cervical cytology samples. &lt;i&gt;Results:&lt;\/i&gt; The HPV genotyping accuracy of both MALDI-TOFMS and SPR was 100% for the HPV genotyping of control materials. In the analysis of the 79 HPV-positive samples by MALDI-TOFMS, HPV positivity was 88.6% (70\/79). Nine samples were non-high-risk HPV (non-HR-HPV), which were not targets of MALDI-TOFMS. In the analysis of the 50 cervical samples, the agreement of both tests was 84% with a \u0138 value of 0.660. By using consensus results that mean agreement between 2 of 3 methods, the HR-HPV genotyping accuracy was 100% (77\/77) by MALDI-TOFMS and 94.8% (73\/77) by SPR in the 129 cervical samples. The sensitivity (88.2%; 82\/93) and specificity (88.9%; 32\/36) of MALDI-TOFMS were similar to those of SPR. &lt;i&gt;Conclusion:&lt;\/i&gt; These results support that MALDI-TOFMS is a sensitive, specific and feasible method for HR-HPV detection in clinical application, compared with the SPR method.<\/jats:p>","DOI":"10.1159\/000322722","type":"journal-article","created":{"date-parts":[[2011,10,10]],"date-time":"2011-10-10T15:26:26Z","timestamp":1318260386000},"page":"326-332","source":"Crossref","is-referenced-by-count":4,"title":["A Comparison of Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry and Surface Plasmon Resonance for Genotyping of High-Risk Human 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