{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T17:08:10Z","timestamp":1747933690211,"version":"3.40.4"},"reference-count":19,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurodegener Dis"],"published-print":{"date-parts":[[2011]]},"abstract":"<i>Background:<\/i> Motor neuron disease or amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the selective death of motor neurons in the spinal cord as well as motor cortex. Recently, vascular endothelial growth factor (VEGF) has been identified as a neurotrophic factor in animal models of familial ALS and other neurological diseases. <i>Objective:<\/i> The present study was designed to investigate the neuroprotective role of VEGF in the more prevalent sporadic form of ALS. <i>Methods:<\/i> We studied the effect of VEGF on the NSC-34 cell line exposed to cerebrospinal fluid (CSF) from sporadic ALS patients (ALS-CSF) in terms of lactate dehydrogenase (LDH) assay as well as choline acetyltransferase (ChAT) and phosphorylated neurofilament expression by immunocytochemistry and confocal microscopy. NSC-34 cells were exposed to CSF from patients with definite ALS and compared to controls. LDH activity was assessed in the growth media, prior to and 24 h after the addition of VEGF to the cells. At similar time points, the cells were fixed and processed for immunocytochemistry to evaluate ChAT and phosphorylated neurofilament expression. <i>Results:<\/i> Exposure to ALS-CSF caused morphological changes of NSC-34 cells like reduced differentiation and aggregation of phosphorylated neurofilaments. Enhanced LDH activity and reduced ChAT immunoreactivity were also observed. Addition of VEGF to NSC-34 cells exposed to ALS-CSF was protective in terms of reduced LDH activity and restoration of ChAT expression. <i>Conclusion:<\/i> The present study confirms that VEGF exerts a neuroprotective effect on the NSC-34 cell line by attenuating the degenerative changes induced by ALS-CSF. It thus has therapeutic potential in sporadic ALS.<\/jats:p>","DOI":"10.1159\/000323718","type":"journal-article","created":{"date-parts":[[2011,3,10]],"date-time":"2011-03-10T08:22:43Z","timestamp":1299745363000},"page":"322-330","source":"Crossref","is-referenced-by-count":18,"title":["Vascular Endothelial Growth Factor Attenuates Neurodegenerative Changes in the NSC-34 Motor Neuron Cell Line Induced by Cerebrospinal Fluid of Sporadic Amyotrophic Lateral Sclerosis Patients"],"prefix":"10.1159","volume":"8","author":[{"given":"Dinkar","family":"Kulshreshtha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Vijayalakshmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phalguni Anand","family":"Alladi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.N.","family":"Sathyaprabha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atchayaram","family":"Nalini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.R.","family":"Raju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2011,3,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097%2F01.wco.0000187248.21103.c5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM200105313442207"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1440-1789.2002.00449.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097%2F00001756-199608120-00022"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006%2Fexnr.1997.6651"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002%2Faja.1001940306"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.brainres.2009.01.041"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1080%2F146608202760196048"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fmus.20609"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038%2F88842"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1097%2F01.jnen.0000196134.51217.74"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1471-4159.2007.05206.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136%2Fjnnp.2006.104828"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jns.2006.12.004"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1471-4159.1989.tb09167.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuint.2004.12.004"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1097%2Fnen.0b013e3181567c16"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.brainresbull.2007.11.018"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1590%2FS0100-879X2005000600015"}],"container-title":["Neurodegenerative Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/323718","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/323718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:24:14Z","timestamp":1745436254000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000323718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":19,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2011,6,1]]},"published-print":{"date-parts":[[2011,6,9]]}},"URL":"https:\/\/doi.org\/10.1159\/000323718","archive":["Portico"],"relation":{},"ISSN":["1660-2854","1660-2862"],"issn-type":[{"type":"print","value":"1660-2854"},{"type":"electronic","value":"1660-2862"}],"subject":[],"published":{"date-parts":[[2011]]}}}