{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:51:40Z","timestamp":1787017900594,"version":"build-2736575974"},"reference-count":25,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Blood Purif"],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Uremic toxins are typically measured in plasma and little is known of their interstitial concentrations. We undertook experiments to validate a microdialysis technique for simultaneous recovery of small and large uremic toxins in the subcutaneous interstitial fluid (ISF). &lt;i&gt;Methods:&lt;\/i&gt; Microdialysis catheters were inserted into the subcutaneous interstitium of 8 subjects (controls and uremic patients) and perfused using two different solutions at incremental flow rates to determine analyte recovery and ISF concentrations of urea and protein. &lt;i&gt;Results:&lt;\/i&gt; 10% dextran-40 perfusate allowed the determination of interstitial concentrations of urea and protein reliably, by virtue of the exponential decay of their concentrations in the microdialysate with incremental flow rates (R&lt;sup&gt;2&lt;\/sup&gt; = 0.63\u20130.99). Interstitial and plasma urea correlated well (r = 0.95), as did interstitial urea from distant anatomical sites (r = 0.96). &lt;i&gt;Conclusion:&lt;\/i&gt; Cutaneous microdialysis with dextran-40 allows measurement of small and large molecule concentrations in ISF, creating an opportunity to characterize ISF in uremia.<\/jats:p>","DOI":"10.1159\/000324207","type":"journal-article","created":{"date-parts":[[2011,3,3]],"date-time":"2011-03-03T03:36:37Z","timestamp":1299123397000},"page":"96-103","source":"Crossref","is-referenced-by-count":11,"title":["A Modified in vivo Flow Variation Technique of Microdialysis for Sampling Uremic Toxins in the Subcutaneous Interstitial Compartment"],"prefix":"10.1159","volume":"32","author":[{"given":"Leonard","family":"Ebah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Brenchley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Coupes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandip","family":"Mitra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2011,3,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00467-008-0762-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.63.s84.43.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfn521"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.atherosclerosis.2009.05.011"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1681%2FASN.2005020132"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1053%2Fj.ajkd.2007.05.009"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fki.1993.290"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa021583"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093%2Fqjmed%2F92.5.251"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000057480"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3171%2Fjns.2000.93.1.0037"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejps.2005.01.017"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1440-1797.2003.00137.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093%2Fndt%2Fgfl721"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1159%2F000110570"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2FS0003-2670%2898%2900598-4"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1021%2Fac9811930"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2F0165-0270%2885%2990107-4"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1023%2FA%3A1018971818689"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1063%2F1.1485111"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2FS0141-8130%2803%2900055-2"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1227%2F01.NEU.0000159711.93592.8D"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01639123"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1208%2Faapsj070369"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00701-005-0670-8"}],"container-title":["Blood Purification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:28:53Z","timestamp":1745422133000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000324207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":25,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2011,8,1]]},"published-print":{"date-parts":[[2011,8,2]]}},"URL":"https:\/\/doi.org\/10.1159\/000324207","archive":["Portico"],"relation":{},"ISSN":["0253-5068","1421-9735"],"issn-type":[{"value":"0253-5068","type":"print"},{"value":"1421-9735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}