{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:41:56Z","timestamp":1791276116515,"version":"4.1.0"},"reference-count":20,"publisher":"S. Karger AG","issue":"2-4","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells Tissues Organs"],"published-print":{"date-parts":[[2011]]},"abstract":"Dentin matrix protein 1 (DMP1) is an acidic protein that plays critical roles in osteogenesis and dentinogenesis. Protein chemistry studies have demonstrated that DMP1 primarily exists as processed NH<sub>2<\/sub>- and COOH-terminal fragments in the extracellular matrix of bone and dentin. Our earlier work showed that the substitution of Asp<sup>213<\/sup> (a residue at a cleavage site) by Ala<sup>213<\/sup> blocks the processing of mouse DMP1 in vitro. Recently, we generated transgenic mice expressing this mutant DMP1 (designated \u2018D213A-DMP1\u2019). By crossbreeding these transgenic mice with <i>Dmp1<\/i>-knockout (<i>Dmp1<\/i>-KO) mice, we obtained mice expressing the D213A-DMP1 transgene in the <i>Dmp1<\/i>-null background (named \u2018<i>Dmp1<\/i>-KO\/<i>D213A-Tg\u2019<\/i> mice). In this study, we analyzed the long bone, mandible, dentin, and cartilage of <i>Dmp1-<\/i>KO\/<i>D213A-Tg<\/i> mice in comparison with wild-type,<i> Dmp1<\/i>-KO, and <i>Dmp1<\/i>-KO mice expressing the normal DMP1 transgene (<i>Dmp1<\/i>-KO\/normal-Tg). Our results showed that D213A-DMP1 was barely cleaved in the dentin matrix of <i>Dmp1<\/i>-KO\/<i>D213A-Tg<\/i> mice and the expression of D213A-DMP1 failed to rescue the developmental defects in <i>Dmp1<\/i>-null mice. Interestingly, enlarged growth plates and condylar cartilages were observed in <i>Dmp1<\/i>-KO\/<i>D213A-Tg<\/i> mice, indicating a potential role of the full-length form of DMP1 in chondrogenesis.<\/jats:p>","DOI":"10.1159\/000324672","type":"journal-article","created":{"date-parts":[[2011,5,9]],"date-time":"2011-05-09T08:17:27Z","timestamp":1304929047000},"page":"199-204","source":"Crossref","is-referenced-by-count":32,"title":["Roles of DMP1 Processing in Osteogenesis, Dentinogenesis and Chondrogenesis"],"prefix":"10.1159","volume":"194","author":[{"given":"Yao","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Q.","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlin","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2011,5,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.matbio.2004.07.008"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1359%2Fjbmr.1997.12.12.2040"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1177%2F154405910308201003"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038%2Fng1905"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1006%2Fgeno.1997.4700"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00223-008-9140-7"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ydbio.2006.11.001"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1359%2Fjbmr.1998.13.3.422"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1369%2Fjhc.2008.952630"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159%2F000151373"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M305315200"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1177%2F154405910708601202"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M512964200"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M310179200"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijom.2009.12.017"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1369%2Fjhc.2010.956771"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M110.137059"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M314114200"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M400490200"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M412911200"}],"container-title":["Cells Tissues Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324672","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324672","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:33:42Z","timestamp":1745436822000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000324672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":20,"journal-issue":{"issue":"2-4","published-online":{"date-parts":[[2011,8,1]]},"published-print":{"date-parts":[[2011,8,10]]}},"URL":"https:\/\/doi.org\/10.1159\/000324672","archive":["Portico"],"relation":{},"ISSN":["1422-6405","1422-6421"],"issn-type":[{"value":"1422-6405","type":"print"},{"value":"1422-6421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}