{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T07:26:27Z","timestamp":1774682787267,"version":"3.50.1"},"reference-count":17,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; We established for the first time the prevalence of &lt;i&gt;Chlamydia trachomatis&lt;\/i&gt; infection among infertile women in Manaus Amazonas Brazil using the polymerase chain reaction (PCR) technique. &lt;i&gt;Methods:&lt;\/i&gt; 106 women were studied at a public university hospital fertility clinic for infertility problems correlated with chlamydia infection. Social-economic and clinical information was obtained before medical examination to obtain samples for the amplification of &lt;i&gt;C. trachomatis&lt;\/i&gt; DNA plasmid. &lt;i&gt;Results:&lt;\/i&gt; The prevalence of chlamydial infection among infertile women was 52.8, and 51.8% of the positive participants were older than 30 years of age (p = 0.8697). Of the 56 women positive for &lt;i&gt;C. trachomatis&lt;\/i&gt;, 31 (55.4%) had never given birth, while 9 (16%) had a pregnancy resulting in fetal death. Our findings also revealed that these patients had a low socio-economic status and high unemployment (p = 0.0274), and a significant association of 5% (p &lt; 0.05) of chlamydial infection with family income. &lt;i&gt;Conclusions:&lt;\/i&gt; Due to the high prevalence of &lt;i&gt;C. trachomatis&lt;\/i&gt;, concerns about effects on reproductive health and fertility are undertaken. Therefore, we believe it is extremely necessary to implement large-scale PCR-based screening as part of routine clinical detection programs concerning preventive effects of chlamydial infection among this population.<\/jats:p>","DOI":"10.1159\/000324798","type":"journal-article","created":{"date-parts":[[2011,12,5]],"date-time":"2011-12-05T08:34:21Z","timestamp":1323074061000},"page":"220-226","source":"Crossref","is-referenced-by-count":10,"title":["High Prevalence Detection of &lt;i&gt;Chlamydia trachomatis&lt;\/i&gt; by Polymerase Chain Reaction in Endocervical Samples of Infertile Women Attending University Hospital in Manaus-Amazonas, Brazil"],"prefix":"10.1159","volume":"72","author":[{"given":"Norma Suely","family":"de Lima Freitas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina Maria","family":"Borborema-Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e1ria","family":"Barroso Serr\u00e3o das Neves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cintia Mara","family":"Costa de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fania Raquel","family":"Dutra Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spartaco","family":"Astolfi-Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2011,11,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bpobgyn.2006.06.003"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000072075"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097%2F01.olq.0000137899.25542.75"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1590%2FS0102-311X2006000200010"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097%2F01.olq.0000258399.27873.d9"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.gyobfe.2004.10.017"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1093%2Fnar%2F25.17.3389"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093%2Fhumupd%2F5.5.433"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.39.5.1751-1756.2001"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0015-0282%2898%2900248-9"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1053%2Fbeog.2002.0319"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1093%2Fhumrep%2F17.3.525"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1186%2F1471-2458-7-95"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000230112"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1471-0528.1998.tb10173.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1053%2Fbeog.2002.0329"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1159%2F000010332"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324798","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324798","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/324798","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:34:57Z","timestamp":1745436897000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000324798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":17,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2011,12,1]]},"published-print":{"date-parts":[[2011,12,6]]}},"URL":"https:\/\/doi.org\/10.1159\/000324798","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"value":"0378-7346","type":"print"},{"value":"1423-002X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}