{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:24:15Z","timestamp":1787876655219,"version":"build-2784847793"},"reference-count":26,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2011]]},"abstract":"Previous studies showed that in the mouse mutant <i>Lis1<\/i><sup>GT\/GT<\/sup> gene trap integration in intron 2 of <i>Lis1<\/i> gene leads to male infertility in homozygous <i>Lis1<\/i><sup>GT\/GT<\/sup> mice. We further analyzed this line and could confirm the suggested downregulation of a testis-specific <i>Lis1<\/i> transcript in mutant animals in a quantitative manner. Moreover, we analyzed the gene trap mutation on different genetic backgrounds in incipient congenic animals and could exclude a genetic background effect. To gain further insights into the role and requirement of LIS1 in spermatogenesis, 3 transgenic lines were generated, that overexpress <i>Lis1<\/i> under control of the testis-specific promoters <i>hEF-1<\/i>\u03b1, which is exclusively active in spermatogonial cells, <i>PGK2<\/i>, which is active in pachytene spermatocytes and following stages of spermatogenesis, and <i>Tnp2<\/i> which is active in round spermatids and following stages of spermatogenesis, respectively. All 3 transgenic lines remained fertile and testis sections displayed no abnormalities. To overcome the infertility of <i>Lis1<\/i><sup>GT\/GT<\/sup> males, these transgenic <i>Lis1<\/i>-overexpressing animals were mated with <i>Lis1<\/i><sup>GT\/GT<\/sup> mice to generate \u2018rescued\u2019 <i>Lis1<\/i><sup>GT\/GT<\/sup><i>\/Lis1<\/i><sup>Tpos<\/sup> males. \u2018Rescued\u2019 animals from all transgenic lines remained infertile, thus overexpression of <i>Lis1<\/i> in different stages of spermatogenesis could not rescue the infertility phenotype of homozygous gene trap males.<\/jats:p>","DOI":"10.1159\/000329482","type":"journal-article","created":{"date-parts":[[2011,8,17]],"date-time":"2011-08-17T06:19:16Z","timestamp":1313561956000},"page":"269-282","source":"Crossref","is-referenced-by-count":4,"title":["Overexpression of <i>Lis1<\/i> in Different Stages of Spermatogenesis Does Not Result in an Aberrant Phenotype"],"prefix":"10.1159","volume":"134","author":[{"given":"N.","family":"Drusenheimer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Nayernia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Meinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.-H.","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Engel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2011,7,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.molbrainres.2004.07.002"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2Fng.302"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2Fmrd.10339"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0012-1606%2883%2990368-8"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.270.38.22308"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2F1221"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.tig.2007.09.003"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2F0012-1606%2884%2990262-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M211836200"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2F0012-1606%2881%2990058-0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1523%2FJNEUROSCI.1609-07.2007"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.139.7.3289"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0076-6879%2804%2989001-3"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0166-2236%2800%2901887-7"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038%2F326501a0"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.287.5457.1489"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2F1098-2795%2820010401%2958%3A4%3C368%3A%3AAID-MRD3%3E3.0.CO%3B2-J"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M309583200"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1006%2Fgeno.1997.5121"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.86.21.8437"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolehr%2F7.12.1123"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1083%2Fjcb.113.6.1385"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.13.19.2538"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1399-0004.2007.00888.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2443.2007.01126.x"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.1236145100"}],"container-title":["Cytogenetic 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