{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T16:28:55Z","timestamp":1756312135760,"version":"3.40.4"},"reference-count":13,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T00:00:00Z","timestamp":1318896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T00:00:00Z","timestamp":1318896000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>&lt;i&gt;Purpose:&lt;\/i&gt; Here we investigate the role of donor endothelium on allograft rejection in a lamellar keratoplasty (LK) model using grafts with or without donor endothelium. &lt;i&gt;Methods:&lt;\/i&gt; Corneal buttons of donor C57BL\/6 mice (2.0 mm) were transplanted to lamellar recipient beds (1.5 mm) in BALB\/c mice. Two variations of the LK procedure were performed: (1) standard LK (SLK) (n = 13) without donor endothelium and (2) modified LK (MLK) (n = 14) with retained donor endothelium. The graft status was assessed by slit lamp biomicroscopy and scored for stromal opacity, corneal edema, neovascularization, and anterior chamber reaction up to 46 days post-transplantation. Corneas were also observed histologically. &lt;i&gt;Results:&lt;\/i&gt; The presence of a grafted corneal endothelium promoted graft rejection; 92.9% (13\/14) of grafts were rejected in MLK after an average of 8.3 days, while 69.2% (9\/13) of grafts were rejected in SLK on average 10.8 days after transplantation. The former\u2019s stromal opacity was significantly greater at all time points after day 14 except for day 21 (p = 0.77) and day 32 (p = 0.25). Corneal edema was significantly greater in the former at all time points after day 10 except for day 21 (p = 0.16). Neovascularization was significantly greater in the former at all time points after day 10 except for day 25 (p = 0.22). &lt;i&gt;Conclusion:&lt;\/i&gt; Variations of this model of LK may be useful for studies of immunological mechanisms in corneal transplantation. The donor corneal endothelium may serve as a target of the immune response which promotes inflammation, neovascularization, and graft rejection.<\/jats:p>","DOI":"10.1159\/000330271","type":"journal-article","created":{"date-parts":[[2011,10,18]],"date-time":"2011-10-18T07:36:34Z","timestamp":1318923394000},"page":"128-134","source":"Crossref","is-referenced-by-count":3,"title":["The Effects of Corneal Endothelium on Graft Survival in a Murine Model of Lamellar Keratoplasty"],"prefix":"10.1159","volume":"47","author":[{"given":"Ting","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen J.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen R.","family":"Planck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James T.","family":"Rosenbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2011,10,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097%2F00003226-200009000-00006"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1080%2F09273940902802683"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.eye.6700615"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI119173"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097%2F00007890-199710270-00004"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1136%2Fbjo.82.3.294"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3109%2F02713689208999545"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1189%2Fjlb.1102543"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000060740"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097%2F00007890-199906270-00001"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159%2F000058740"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1080%2F08830180212064"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1167%2Fiovs.04-0144"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/330271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/330271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:27:27Z","timestamp":1745432847000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000330271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,18]]},"references-count":13,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,1]]},"published-print":{"date-parts":[[2012,3,23]]}},"URL":"https:\/\/doi.org\/10.1159\/000330271","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"type":"print","value":"0030-3747"},{"type":"electronic","value":"1423-0259"}],"subject":[],"published":{"date-parts":[[2011,10,18]]}}}