{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:34:24Z","timestamp":1745469264189,"version":"3.40.4"},"reference-count":7,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2011,10,7]],"date-time":"2011-10-07T00:00:00Z","timestamp":1317945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2011,10,7]],"date-time":"2011-10-07T00:00:00Z","timestamp":1317945600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Chromosomal abnormalities, like deletions, amplifications, inversions or translocations, are recurrent features in haematological malignancies. However, the precise molecular breakpoints are frequently not determined. Here we describe a rapid analysis of genetic imbalances combining fine tiling comparative genomic hybridization (FT-CGH) and ligation-mediated PCR (LM-PCR). We clarified an inv(14)(q11q32) in a case of T cell acute lymphoblastic leukaemia with a breakpoint in the TRA\/D in 68% of cells detected by fluorescence in situ hybridization. FT-CGH showed several mono- and biallelic losses within TRA\/D. LM-PCR disclosed a TRA\/D rearrangement on one allele. The other allele revealed an inv(14)(q11q32), joining TRDD2 at 21,977,000 of 14q11 together with the IGH locus at 105,948,000 and 3\u2032-sequence of TRAC at 22,092,000 joined together with IGHV4\u201361 at 106,166,000. This sensitive approach can unravel complex chromosomal abnormalities in patient samples with a limited amount of aberrant cells and may lead to better diagnostic and therapeutic options.<\/jats:p>","DOI":"10.1159\/000330519","type":"journal-article","created":{"date-parts":[[2011,10,10]],"date-time":"2011-10-10T15:26:26Z","timestamp":1318260386000},"page":"16-19","source":"Crossref","is-referenced-by-count":3,"title":["Fast Approach for Clarification of Chromosomal Aberrations by Using LM-PCR and FT-CGH in Leukaemic Sample"],"prefix":"10.1159","volume":"127","author":[{"given":"Kathleen","family":"Dittmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grzegorz Krzysztof","family":"Przybylski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piotr","family":"Grabarczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gottfried","family":"D\u00f6lken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Gesk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reiner","family":"Siebert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian Andreas","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2011,10,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2402884"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002%2Fpath.2196"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.85.23.9287"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2141.2000.02256.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2403619"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.leu.2402387"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-0609.2010.01508.x"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/aha\/article-pdf\/127\/1\/16\/3892746\/000330519.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/aha\/article-pdf\/127\/1\/16\/3892746\/000330519.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:30:08Z","timestamp":1745433008000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000330519"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,7]]},"references-count":7,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2011,12,1]]},"published-print":{"date-parts":[[2011,12,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000330519","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5122690","asserted-by":"subject"}]},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"type":"print","value":"0001-5792"},{"type":"electronic","value":"1421-9662"}],"subject":[],"published":{"date-parts":[[2011,10,7]]}}}