{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:10:36Z","timestamp":1761513036032,"version":"3.40.4"},"reference-count":43,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2011]]},"abstract":"<i>Objective:<\/i> This study evaluated the usefulness of p16<sup>INK4a<\/sup>\/Ki-67 as a new biomarker in the diagnosis of human papillomavirus (HPV)-related cervical lesions. <i>Study Design:<\/i> From 69 women with previous positive cytology, clinician-collected (CC) samples were obtained using a Cervex-Brush\u00ae. One month later, self-collected (SC) material was acquired using a Rovers\u00ae Viba-Brush. Liquid-based cytology specimens were prepared from both samples, and then the grades of squamous intraepithelial lesions (SIL) were determined; following immunostaining with CINtec\u00ae PLUS, HPV status was analyzed using a linear array. <i>Results:<\/i> The mean double-positive cell scores (SCORE) in the CC samples were 3.2 in samples negative for intraepithelial lesions or malignancy, 1.3 in atypical squamous cells of undetermined significance, 87.1 in low-grade SIL, and 367 in high-grade SIL. According to HPV risk type, the mean SCORE was 16 in samples negative for HPV, 8.4 in low-risk HPV, 143 in high-risk HPV other than type 16, and 420 in HPV16 \u2013 with a statistical significance between high-risk HPV and type 16 (p < 0.05). The SCORE of the SC group was lower than that of the CC group because of the limited number of cells collected. <i>Conclusions:<\/i> The SCORE showed a significant correlation with HPV16 compared with lesser-degree lesions. CINtec PLUS is useful as a diagnostic marker of progression of lesions and a surrogate marker of an elevated risk of high-grade lesions with HPV16.<\/jats:p>","DOI":"10.1159\/000331047","type":"journal-article","created":{"date-parts":[[2011,10,10]],"date-time":"2011-10-10T15:26:26Z","timestamp":1318260386000},"page":"413-420","source":"Crossref","is-referenced-by-count":20,"title":["Usefulness of CINtec\u00ae PLUS p16INK4a\/Ki-67 Double-Staining in Cytological Screening of Cervical 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