{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:42:28Z","timestamp":1792183348354,"version":"4.4.0"},"reference-count":23,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2011,11,26]],"date-time":"2011-11-26T00:00:00Z","timestamp":1322265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,11,26]],"date-time":"2011-11-26T00:00:00Z","timestamp":1322265600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2012]]},"abstract":"<i>Objectives:<\/i> The function of netrin-1 in pathological angiogenesis and its role in retinal neovascularization were investigated in the retinas of oxygen-induced retinopathy (OIR) mice by inhibition of netrin-1. <i>Methods:<\/i> Expression of netrin-1 mRNA and protein in the retinas of OIR mice was analyzed by quantitative RT-PCR and immunoblotting. Inhibition of retinal neovascularization was achieved by lentivirus-mediated netrin-1 small hairpin RNA (shRNA) infection. Retinal neovascularization was examined by fluorescein angiography and quantification of preretinal neovascular nuclei in retinal sections. <i>Results:<\/i> Both mRNA and protein expression of netrin-1 were significantly upregulated in postnatal day 17 OIR mouse retinas. Treatment of OIR mice with specific lentivirus-mediated netrin-1 shRNA dramatically reduced neovascular outgrowth into the inner limiting membrane. Neovascular tufts and nonperfused areas were also reduced. <i>Conclusions:<\/i> High expression of netrin-1 was detected in the retina under ischemic conditions and played a significant role in pathological retinal angiogenesis. Therefore, netrin-1 represents a potential therapeutic target for diabetic retinopathy, retinopathy of prematurity and other ocular neovascular diseases.<\/jats:p>","DOI":"10.1159\/000331428","type":"journal-article","created":{"date-parts":[[2011,11,28]],"date-time":"2011-11-28T10:12:22Z","timestamp":1322475142000},"page":"163-169","source":"Crossref","is-referenced-by-count":15,"title":["Suppression of Retinal Neovascularization by Lentivirus-Mediated Netrin-1 Small Hairpin RNA"],"prefix":"10.1159","volume":"47","author":[{"given":"Huizhuo","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaolian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-zheng","family":"Le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2011,11,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa041773"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1001%2Farchopht.1995.01100120068012"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.0953-816X.2000.01326.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1080%2F02713680802416670"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1124704"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.437807"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature03080"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0405984101"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.coph.2008.11.006"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2FS0896-6273%2800%2980373-6"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.semcdb.2003.09.006"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000324474"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1006%2Fexnr.2000.7589"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1089%2F104303402320987888"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.addr.2009.03.004"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.micinf.2003.10.009"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1158%2F1535-7163.MCT-06-0557"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.devcel.2009.02.006"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0511011103"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.4161%2Fcc.9.8.11197"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10456-008-9096-2"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ydbio.2008.03.023"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1101%2Fgad.437807"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/331428","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/331428","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:39:41Z","timestamp":1745433581000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000331428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,26]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,3,1]]},"published-print":{"date-parts":[[2012,3,23]]}},"URL":"https:\/\/doi.org\/10.1159\/000331428","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"value":"0030-3747","type":"print"},{"value":"1423-0259","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,26]]}}}