{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:14:49Z","timestamp":1791101689387,"version":"4.1.0"},"reference-count":56,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Sexual differentiation of brain function is regulated by estrogen in the perinatal period of rodents. However, the role of the estrogen receptor subtypes ER\u03b1 and ER\u03b2 is still in question. Accordingly, the effects of neonatal treatment with the ER\u03b1 agonist propyl pyrazole triol (PPT) or the ER\u03b2 agonist diarylpropionitrile (DPN) on female reproductive functions were investigated in rats. Female rats were injected subcutaneously with 100\u2013500 \u00b5g\/10 g body weight (b.w.) PPT or DPN, 100 \u00b5g\/10 g b.w. estradiol (E&lt;sub&gt;2&lt;\/sub&gt;), or saline at day 5 (birth day = day 1), and then vaginal opening and vaginal smears were examined. On day 60, their ovaries were removed and lordosis behavior was observed after subcutaneous implantation of a silicon tube containing E&lt;sub&gt;2&lt;\/sub&gt;. As a result, in most PPT and all E&lt;sub&gt;2&lt;\/sub&gt; rats, vaginal opening was advanced and an irregular estrous cycle was observed. In contrast, in most rats of the DPN groups, vaginal opening was comparable to that of the control and there was a regular estrous cycle. Lordosis tests revealed that the mean lordosis quotients (LQs) in the 250- and 500-\u00b5g PPT groups was lower than in the saline group, but higher than in the E&lt;sub&gt;2&lt;\/sub&gt; group. Mean LQs in all DPN groups were comparable to those in the saline group. These results suggest that ER\u03b1 plays a major role in masculinization of the system regulating the estrous cycle in the rat brain. In behavioral defeminization of the lordosis-regulation system, ER\u03b1 was also found to be the main target of estrogen.<\/jats:p>","DOI":"10.1159\/000332128","type":"journal-article","created":{"date-parts":[[2012,2,7]],"date-time":"2012-02-07T08:16:07Z","timestamp":1328602567000},"page":"297-304","source":"Crossref","is-referenced-by-count":7,"title":["Defeminization of Brain Functions by a Single Injection of Estrogen Receptor \u03b1 or \u03b2 Agonist in Neonatal Female Rats"],"prefix":"10.1159","volume":"95","author":[{"given":"Moeko","family":"Kanaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korehito","family":"Yamanouchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2012,2,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1210%2Fendo-68-1-68"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1677%2Fjoe.0.0760311"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.brainres.2005.06.082"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002%2F1096-9861%2820010312%29431%3A3%3C293%3A%3AAID-CNE1071%3E3.0.CO%3B2-M"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1262%2Fjrd.49.67"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159%2F000121961"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.6163211"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.141.3577.273"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1507%2Fendocrj1954.11.204"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2F0018-506X%2875%2990004-5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0018-506X%2877%2990060-5"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1677%2Fjoe.0.0740375"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2F0018-506X%2871%2990014-6"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02137314"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1677%2Fjoe.0.0550455"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038%2Fnn1624"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016%2F0166-2236%2881%2990091-6"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.93.12.5925"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.139.12.5070"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2FS0006-8993%2899%2901452-3"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2826.2003.01089.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.96.22.12887"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0500752102"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2005.10.018"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.139.10.4252"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016%2FS0018-506X%2803%2900122-3"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.brainresbull.2004.10.002"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016%2FS0739-7240%2803%2900047-X"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.tox.2008.09.006"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1095%2Fbiolreprod.109.078261"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016%2FS0303-7207%2803%2900255-7"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1002%2F%28SICI%291096-9861%2819971208%29389%3A1%3C81%3A%3AAID-CNE6%3E3.0.CO%3B2-A"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1002%2Fcne.902120203"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.2183%2Fpjab.62.408"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1007%2Fs11154-007-9032-6"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.2006-0787"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.2006-1540"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.94.25.14077"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.2005-0834"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1523%2FJNEUROSCI.1618-06.2006"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1262%2Fjrd.18146"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.052707299"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1210%2Fendo-126-2-695"},{"key":"ref44","doi-asserted-by":"publisher","DOI":"10.1006%2Ffrne.2001.0219"},{"key":"ref45","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.141.9.3506"},{"key":"ref46","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.142.7.3261"},{"key":"ref47","doi-asserted-by":"publisher","DOI":"10.1095%2Fbiolreprod.108.074583"},{"key":"ref48","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.272.32.19858"},{"key":"ref49","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.143.1.285"},{"key":"ref50","doi-asserted-by":"publisher","DOI":"10.1159%2F000073706"},{"key":"ref51","doi-asserted-by":"publisher","DOI":"10.1016%2F0018-506X%2875%2990023-9"},{"key":"ref52","doi-asserted-by":"publisher","DOI":"10.1016%2F0006-8993%2877%2990544-3"},{"key":"ref53","doi-asserted-by":"publisher","DOI":"10.1159%2F000054448"},{"key":"ref54","doi-asserted-by":"publisher","DOI":"10.1016%2F0306-4530%2877%2990012-9"},{"key":"ref55","doi-asserted-by":"publisher","DOI":"10.1677%2Fjme.0.0280087"},{"key":"ref56","doi-asserted-by":"publisher","DOI":"10.2131%2Fjts.30.75"}],"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/332128","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/332128","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/332128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:46:43Z","timestamp":1745434003000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000332128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":56,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2012,6,1]]},"published-print":{"date-parts":[[2012,6,15]]}},"URL":"https:\/\/doi.org\/10.1159\/000332128","archive":["Portico"],"relation":{},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}