{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:33:36Z","timestamp":1791318816707,"version":"4.3.1"},"reference-count":40,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2011,11,17]],"date-time":"2011-11-17T00:00:00Z","timestamp":1321488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,11,17]],"date-time":"2011-11-17T00:00:00Z","timestamp":1321488000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Neutrophils and oxidative stress have been implicated in the pathogenesis of COPD. Severe, early-onset COPD is characterized by a rapid decline in the lung function at an early age; however, nothing is known about neutrophil activation in COPD patients. &lt;i&gt;Objectives:&lt;\/i&gt; The aim of this study was to evaluate peripheral blood neutrophil activation in severe, early-onset COPD patients versus healthy non-smokers and the effect of N-acetyl-&lt;i&gt;L&lt;\/i&gt;-cysteine (NAC) on neutrophil activation in vitro. &lt;i&gt;Methods:&lt;\/i&gt; Neutrophils were isolated from 15 severe, early-onset COPD patients and 15 age-matched healthy subjects and stimulated with N-formyl- Met-Leu-Phe (fMLP) in the presence or absence of NAC (10 \u00b5&lt;i&gt;M&lt;\/i&gt; to 10 m&lt;i&gt;M&lt;\/i&gt;). Neutrophil chemotaxis, elastase release, reactive oxygen species (ROS), intracellular thiols and apoptosis were measured by Boyden chamber, spectrofluorometry, CMFDA and H&lt;sub&gt;2&lt;\/sub&gt;DCF-DA dyes and by annexin V-FITC, respectively. &lt;i&gt;Results:&lt;\/i&gt; Chemotaxis of peripheral blood neutrophils from COPD patients in response to fMLP was 30% more increased than that observed in healthy subjects. Elastase release in response to fMLP was 2-fold higher in neutrophils from COPD patients versus healthy subjects. Intracellular thiol levels were 30% lower in COPD and ROS was approximately 30% higher in COPD versus healthy neutrophils. Spontaneous apoptosis showed no differences in both groups of patients and fMLP-induced apoptosis was higher in COPD. Pre-treatment with the antioxidant NAC effectively attenuated neutrophil chemotaxis, elastase release and ROS as well as effectively increased thiol levels in COPD. &lt;i&gt;Conclusions:&lt;\/i&gt; Neutrophils in severe, early-onset COPD patients are highly activated and this is alleviated by NAC in vitro.<\/jats:p>","DOI":"10.1159\/000332834","type":"journal-article","created":{"date-parts":[[2011,11,21]],"date-time":"2011-11-21T07:46:48Z","timestamp":1321861608000},"page":"147-158","source":"Crossref","is-referenced-by-count":32,"title":["Neutrophil Activation in Severe, Early-Onset COPD Patients versus Healthy Non-Smoker Subjects in vitro: Effects of Antioxidant 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