{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T11:41:41Z","timestamp":1789818101999,"version":"4.0.1"},"reference-count":14,"publisher":"S. Karger AG","issue":"1-4","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurodegener Dis"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; Induction of a humoral response against amyloid-\u03b2 peptide may be beneficial for Alzheimer\u2019s disease (AD) patients and may alleviate the onset and progression of AD. DNA-based vaccination provides a unique alternative method of immunization for treatment and prevention of AD. Currently, the two major delivery methods used for enhancing DNA uptake and immune responses to DNA vaccines in humans are electroporation (EP) and gene gun (GG). &lt;i&gt;Objective:&lt;\/i&gt; The goal of this translational study was to evaluate the efficacy of an AD DNA epitope vaccine (DepVac) delivered intramuscularly by EP or intradermally by GG. &lt;i&gt;Methods:&lt;\/i&gt; Humoral and cellular immune responses to immunization with DepVac were evaluated by ELISA and ELISPOT, respectively. Functional activity of the antibodies was also assessed. &lt;i&gt;Results:&lt;\/i&gt; EP- and GG-mediated immunizations with DepVac induced similar anti-amyloid-\u03b2 (A\u03b2) antibody and T cell responses. Anti-A\u03b2 antibodies bound to amyloid plaques in AD brain tissue and to toxic forms of A\u03b2&lt;sub&gt;42&lt;\/sub&gt; peptide. &lt;i&gt;Conclusion:&lt;\/i&gt; Both delivery methods are effective at promoting potent antibodies specific for A\u03b2.<\/jats:p>","DOI":"10.1159\/000333359","type":"journal-article","created":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T08:41:11Z","timestamp":1328085671000},"page":"261-264","source":"Crossref","is-referenced-by-count":18,"title":["Delivery of a DNA Vaccine for Alzheimer\u2019s Disease by Electroporation versus Gene Gun Generates Potent and Similar Immune Responses"],"prefix":"10.1159","volume":"10","author":[{"given":"Hayk","family":"Davtyan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anahit","family":"Ghochikyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Movsesyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Ellefsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Petrushina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Cribbs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drew","family":"Hannaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire F.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Agadjanyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2012,2,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.2174%2F187152709787601849"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038%2Fnrneurol.2009.219"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1212%2F01.WNL.0000159740.16984.3C"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1385%2FNMM%3A7%3A3%3A255"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1750-3639.2008.00134.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1523%2FJNEUROSCI.3201-07.2007"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fgt.2009.140"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1586%2Ferv.10.42"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.vaccine.2009.02.061"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.vaccine.2009.10.111"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.vaccine.2009.07.005"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.vaccine.2008.03.064"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS0304-3940%2801%2901951-6"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2FS0165-5728%2802%2900307-7"}],"container-title":["Neurodegenerative Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/333359","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/333359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:39:28Z","timestamp":1745433568000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000333359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":14,"journal-issue":{"issue":"1-4","published-online":{"date-parts":[[2012,4,1]]},"published-print":{"date-parts":[[2012,4,19]]}},"URL":"https:\/\/doi.org\/10.1159\/000333359","archive":["Portico"],"relation":{},"ISSN":["1660-2854","1660-2862"],"issn-type":[{"value":"1660-2854","type":"print"},{"value":"1660-2862","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}