{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:47:15Z","timestamp":1784630835126,"version":"3.55.0"},"reference-count":18,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2011]]},"abstract":"<i>Objective:<\/i> To detect <i>BRAF<\/i> V600E mutation in thyroid fine-needle aspiration (FNA) slides and needle rinses (NR). <i>Study Design:<\/i> Tumor-enriched DNA was extracted from FNA smears, formalin-fixed paraffin-embedded (FFPE) sections, or NR specimens from 37 patients with confirmed papillary thyroid carcinoma or benign findings. An allele-specific primer selectively amplified the 1799 T>A <i>BRAF<\/i> mutation while simultaneously blocking amplification of wild-type (WT) <i>BRAF<\/i> with an unlabeled probe during PCR. Mutation detection was accomplished by melting analysis of the probe. <i>Results:<\/i> Allele-specific\/blocking probe PCR confirmed the <i>BRAF<\/i> mutation status for 20 of 24 paired FNA\/FFPE samples previously tested by fluorescent probe real-time PCR. For the other 4 cases, the sensitive PCR method detected the <i>BRAF<\/i> mutation in all paired FNA\/FFPE samples. Previously, the mutation had been detected in only the FFPE samples. The <i>BRAF<\/i> mutation was also detected in some NR specimens. <i>Conclusion:<\/i> Treatment of patients with thyroid nodules is guided by FNA biopsy, which can be scantly cellular, necessitating a sensitive test that can detect low levels of <i>BRAF<\/i> V600E mutation in a WT background. We report increased detection of <i>BRAF<\/i> V600E in FNA specimens using allele-specific\/blocking probe PCR, which has an analytical sensitivity of 0.01%.<\/jats:p>","DOI":"10.1159\/000333453","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T08:20:51Z","timestamp":1323418851000},"page":"576-583","source":"Crossref","is-referenced-by-count":14,"title":["Allele-Specific PCR with Competitive Probe Blocking for Sensitive and Specific Detection of <i>BRAF<\/i> V600E in Thyroid Fine-Needle Aspiration Specimens"],"prefix":"10.1159","volume":"55","author":[{"given":"G. Denice","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luming","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie R.","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke A.","family":"Jarboe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian T.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel S.","family":"Bentz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl T.","family":"Wittwer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara E.","family":"Chadwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2011,12,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1186%2F1742-6413-3-10"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.2006.040105"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038%2Fmodpathol.2010.167"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.2003-030838"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.mce.2009.10.012"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.2009-2332"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.2007-1184"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjc.6603520"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1158%2F0008-5472.CAN-08-1430"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1186%2F1756-9966-29-132"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1309%2FAJCPO3F2ENKMDTUS"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.2353%2Fjmoldx.2010.100018"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jmoldx.2010.09.005"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1373%2Fclinchem.2009.142034"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.2007.051425"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.2007.052688"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.2353%2Fjmoldx.2010.090238"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.surg.2009.09.011"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/333453","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/333453","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:39:50Z","timestamp":1745433590000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000333453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":18,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2011,12,1]]},"published-print":{"date-parts":[[2011,12,19]]}},"URL":"https:\/\/doi.org\/10.1159\/000333453","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}