{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:00:25Z","timestamp":1791126025693,"version":"4.1.0"},"reference-count":43,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T00:00:00Z","timestamp":1327968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"},{"start":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T00:00:00Z","timestamp":1327968000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Extra"],"abstract":"<jats:p>&lt;i&gt;Background:&lt;\/i&gt; We previously showed that angiotensin type 1 receptor (AT1) blocker (ARB) attenuates glomerular injury in &lt;i&gt;Nphs1-hCD25&lt;\/i&gt; (NEP25) transgenic mice, a model of selective podocyte injury. However, subsequent studies in NEP25 mice with podocyte-specific deficiency of AT1 revealed that the protective effects of ARB are not through the podocyte AT1, thereby raising the possibility that the protective effects of ARB involve mineralocorticoids. &lt;i&gt;Methods:&lt;\/i&gt; NEP25 mice were treated with the mineralocorticoid receptor blocker (MRB) spironolactone (25 mg\/kg\/day, n = 10), the ARB losartan (250 mg\/kg\/day, n = 11), both (ARB+MRB, n = 8) or vehicle (Vehicle, n = 9) from day \u20137 to day 9 of induction of podocyte injury. &lt;i&gt;Results:&lt;\/i&gt; Although MRB did not reduce systolic blood pressure or proteinuria, addition of MRB to ARB significantly attenuated glomerulosclerosis (glomerulosclerosis index: ARB+MRB 1.67 \u00b1 0.19 vs. MRB 2.01 \u00b1 0.29, ARB 2.35 \u00b1 0.19, and Vehicle 2.25 \u00b1 0.26, p &lt; 0.05) and preserved the number of WT1-positive podocytes (ARB+MRB 152.5 \u00b1 9.7 vs. MRB 117.2 \u00b1 9.0 or ARB 113.6 \u00b1 7.4, and ARB+MRB vs. Vehicle 97.5 \u00b1 4.0 per glomerulus; p &lt; 0.05). &lt;i&gt;Conclusion:&lt;\/i&gt; These data suggest that, while MRB does not attenuate proteinuria caused by podocyte-specific injury, it provides protective effects against glomerulosclerosis that is independent of systemic blood pressure.<\/jats:p>","DOI":"10.1159\/000334961","type":"journal-article","created":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T09:49:56Z","timestamp":1328003396000},"page":"17-26","source":"Crossref","is-referenced-by-count":12,"title":["Mineralocorticoid Receptor Blocker Protects against Podocyte-Dependent Glomerulosclerosis"],"prefix":"10.1159","volume":"2","author":[{"given":"Nobuaki","family":"Takagi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takakuni","family":"Tanizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Kon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes 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