{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:35:53Z","timestamp":1745469353780,"version":"3.40.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2012]]},"abstract":"<i>Aim:<\/i> Our study aimed to investigate the effect of amlodipine on bone metabolism in orchidectomized rats. <i>Methods:<\/i> Eight-week-old rats were divided into three groups. The sham-operated control group (SHAM) and the control group after orchidectomy (ORX) received the standard laboratory diet (SLD). The experimental group after orchidectomy (ORX+AML) received SLD enriched with amlodipine for 12 weeks. Bone marker concentrations in serum of PINP, OPG and IGF-1, and the levels of CTX-I, BAP and BMP-2 in a bone homogenate were measured using enzyme-linked immunosorbent assay. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry. The femurs were used for biomechanical testing. <i>Results:<\/i> Bone markers (CTX-I, BAP, BMP-2) in ORX were higher versus SHAM. In ORX+AML there was a decrease in PINP, CTX-I, BAP, BMP-2 and OPG versus ORX. IGF-1 was decreased in ORX versus SHAM. In ORX+AML it was increased versus ORX. In ORX, a decrease was demonstrated versus SHAM in BMD of the whole body, in the lumbar vertebrae and in both femurs. In ORX+AML there was an increase in BMD of the whole body versus ORX. Three-point bending test revealed a decrease in maximal load values in ORX versus SHAM. After amlodipine administration there was an increase in the left femur versus ORX. <i>Conclusions:<\/i> Amlodipine is capable of mitigating the negative effects of orchidectomy and could be a good prevention of osteoporosis.<\/jats:p>","DOI":"10.1159\/000335491","type":"journal-article","created":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T08:41:03Z","timestamp":1328085663000},"page":"37-43","source":"Crossref","is-referenced-by-count":11,"title":["Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy"],"prefix":"10.1159","volume":"89","author":[{"given":"Iveta","family":"Gradosova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Zivna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Palicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sona","family":"Hubena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klara","family":"Svejkovska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel","family":"Zivny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,2,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1248%2Fbpb.24.628"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2007.09.027"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2010.02.039"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10006-010-0237-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2F0014-2999%2892%2990005-O"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1359%2FJBMR.050521"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jbspin.2004.04.004"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1210%2Fer.23.3.279"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.87.4.1443"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02408524"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007%2FBF02553294"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.141.5.1642"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1677%2FJME-09-0048"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1677%2Fjoe.0.1710425"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002%2Fjcb.20201"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature01658"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.273.42.27091"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1006%2Fbbrc.1998.9394"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M006918200"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2796.1992.tb00550.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1210%2Fjc.83.7.2576"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bone.2005.06.016"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1093%2Fgerona%2F59.12.1285"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00223-003-0090-9"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1210%2Fen.135.3.996"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.2174%2F1381612043383764"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/335491","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/335491","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:58:00Z","timestamp":1745434680000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000335491"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":26,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2012,3,1]]},"published-print":{"date-parts":[[2012,3,19]]}},"URL":"https:\/\/doi.org\/10.1159\/000335491","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"type":"print","value":"0031-7012"},{"type":"electronic","value":"1423-0313"}],"subject":[],"published":{"date-parts":[[2012]]}}}