{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:01:22Z","timestamp":1791644482950,"version":"4.3.4"},"reference-count":28,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2012]]},"abstract":"<b><i>Background:<\/i><\/b> To investigate the role of efflux pumps AdeABC, AdeIJK, AdeDE and AbeM in reduced susceptibility to imipenem in clinical isolates of <i>Acinetobacter baumannii<\/i>. <b><i>Methods:<\/i><\/b> Susceptibility testing was conducted by the agar dilution method. The presence and expression of pump genes were analyzed by conventional PCR and real-time RT-PCR, respectively. <b><i>Results:<\/i><\/b> Most antibiotics, including carbapenem, aminoglycosides, fluoroquinolones and cephalosporins showed high minimum inhibitory concentration (MIC) values in imipenem-resistant <i>A. baumannii<\/i> (IRAB). After exposure to the efflux pump inhibitor Phe-Arg-\u03b2-naphthylamide, a 4- to 32-fold reduction of imipenem MICs was observed in 33 (66%) isolates of IRAB, while no significant decrease occurred in imipenem-susceptible <i>A. baumannii<\/i> (ISAB). The majority of IRAB isolates (>80%) were found to be positive for <i>adeB<\/i>, <i>adeR<\/i>, <i>adeS<\/i>, <i>adeJ<\/i> and <i>abeM<\/i>. Positive <i>adeE<\/i> was discovered in 6 isolates of IRAB and coexisted with <i>adeB<\/i>. Approximate 4-, 3- and 11-fold increases in <i>adeB<\/i>, <i>adeJ<\/i> and <i>abeM<\/i> expression were observed, respectively, in IRAB compared to those in ISAB. <b><i>Conclusions:<\/i><\/b> A variety of efflux pump systems, including AdeABC, AdeIJK and AbeM, seem to play important roles in the resistance to imipenem of <i>A. baumannii<\/i>. Moreover, this is the first time that <i>adeE<\/i> has been found to coexist with <i>adeB<\/i> in a small number of isolates of <i>A. baumannii<\/i>.<\/jats:p>","DOI":"10.1159\/000335599","type":"journal-article","created":{"date-parts":[[2012,5,21]],"date-time":"2012-05-21T13:11:18Z","timestamp":1337605878000},"page":"152-158","source":"Crossref","is-referenced-by-count":63,"title":["Study of the Correlation of Imipenem Resistance with Efflux Pumps AdeABC, AdeIJK, AdeDE and AbeM in Clinical Isolates of <b><i>Acinetobacter baumannii<\/i><\/b>"],"prefix":"10.1159","volume":"58","author":[{"given":"Pan Fei","family":"Hou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang Feng","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya Ping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun Mei","family":"Ying","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2012,5,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1086%2F502205"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-0691.2005.01184.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1128%2FCMR.00058-07"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMra070741"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.45.12.3375-3380.2001"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkq218"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.00732-07"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.48.10.4054-4055.2004"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.49.10.4362-4364.2005"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1099%2Fjmm.0.46433-0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.sbi.2004.10.003"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2958.2000.01926.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.biochem.73.011303.074104"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.48.9.3298-3304.2004"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2F49.4.697"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159%2F000323620"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.01684-07"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2FS0140-6736%2894%2990694-7"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-0691.2005.01096.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.00699-06"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.01464-06"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.01512-06"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159%2F000319903"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.00343-07"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1159%2F000086932"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016%2FS0968-0004%2801%2901852-7"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.01198-06"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijantimicag.2008.06.027"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/335599","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/335599","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/335599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:58:45Z","timestamp":1745434725000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000335599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2012,6,1]]},"published-print":{"date-parts":[[2012,6,18]]}},"URL":"https:\/\/doi.org\/10.1159\/000335599","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"value":"0009-3157","type":"print"},{"value":"1421-9794","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}