{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T14:30:13Z","timestamp":1778682613615,"version":"3.51.4"},"reference-count":6,"publisher":"S. Karger AG","issue":"3-4","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Dipfluzine hydrochloride (Dip), a novel diphenylpiperazine calcium channel blocker, has revealed the characteristics of a promising candidate for the treatment of cerebral vascular diseases in preclinical studies. Our research identified and quantified Dip and its 4 metabolites (M1, M2, M4 and M5) in rat liver microsomes by liquid chromatography tandem mass spectrometry. The results showed that Dip was firstly metabolized to M1 and M5 by 1- and 4-dealkylation from a piperazine nitrogen, and then the latter was subsequently metabolized to M2 and M4. The concentrations of Dip, M1, M2 and M5 were 557.3 \u00b1 26.3, 854.3 \u00b1 46.0, 2796.7\u00b1 126.9, 2473.3 \u00b1 82.6 and 4.0 \u00b1 0.4, 2.4 \u00b1 0.1, 318.2 \u00b1 8.7 and 27.4 \u00b1 1.5 ng\/ml in male and female rats, respectively. M4 (404.2 \u00b1 22.2 ng\/ml) was detected only in males not in females, suggesting that there is gender difference in the metabolism of Dip.<\/jats:p>","DOI":"10.1159\/000337036","type":"journal-article","created":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T07:46:27Z","timestamp":1334735187000},"page":"201-210","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous Determination of a Novel Diphenylpiperazine Calcium Channel Blocker and Its Four Metabolites in Rat Liver Microsomes by Liquid Chromatography Tandem Mass Spectrometry"],"prefix":"10.1159","volume":"89","author":[{"given":"Wei","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhi","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingfeng","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,3,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1161%2FCIRCULATIONAHA.107.187998"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2FS0003-9993%2895%2980567-2"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2FS0003-9993%2895%2980568-0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.2174%2F1389200013338504"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.yrtph.2009.02.007"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1124%2Fdmd.31.7.924"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/337036","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/337036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T19:11:40Z","timestamp":1745435500000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000337036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":6,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2012,4,1]]},"published-print":{"date-parts":[[2012,4,30]]}},"URL":"https:\/\/doi.org\/10.1159\/000337036","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}