{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:44:41Z","timestamp":1790700281592,"version":"4.1.0"},"reference-count":28,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2012]]},"abstract":"<i>Background\/Aims:<\/i> The activation of proteinase-activated receptors (PARs) has been implicated in the development of important hallmarks of inflammation, including in vivo leukocyte recruitment. Here, we examined the effects of aprotinin, a potent inhibitor of trypsin proteinase and the kallikrein-kinin system, and the PAR-4 antagonist YPGKF-NH<sub>2<\/sub> (tcY-NH<sub>2<\/sub>) on neutrophil recruitment in response to carrageenan and trypsin in the pleural cavity of mice. <i>Methods:<\/i> BALB\/c mice were intrapleurally injected with trypsin or PAR-4-activating peptide AY-NH<sub>2<\/sub>, pretreated with aprotinin or tcY-NH<sub>2<\/sub> (1 \u00b5g\/cavity) prior to an intrapleural injection of trypsin or carrageenan, or pretreated with leukotriene B<sub>4<\/sub> antagonist U-75302 (3 \u00b5g\/cavity) prior to a trypsin injection. The number of infiltrating neutrophils was evaluated after 4 h. <i>Results:<\/i> PAR-4-activating peptide AY-NH<sub>2<\/sub> and trypsin-induced neutrophil recruitment was inhibited by aprotinin, tcY-NH<sub>2<\/sub> or U-75302. Aprotinin and tcY-NH<sub>2<\/sub> also inhibited neutrophil recruitment induced by carrageenan. <i>Conclusion:<\/i> These data suggest a key role for PAR-4 in mediating neutrophil recruitment in a mouse model of pleurisy induced by the activity of trypsin or trypsin-like enzymes.<\/jats:p>","DOI":"10.1159\/000337378","type":"journal-article","created":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T03:46:27Z","timestamp":1334720787000},"page":"275-282","source":"Crossref","is-referenced-by-count":16,"title":["Proteinase-Activated Receptor-4 Plays a Major Role in the Recruitment of Neutrophils Induced by Trypsin or Carrageenan during Pleurisy in Mice"],"prefix":"10.1159","volume":"89","author":[{"given":"L.F.","family":"Gomides","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.D.","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.G.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.","family":"Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.N.","family":"Francischi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2012,4,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.micinf.2003.09.008"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1124%2Fpr.110.002733"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2Fwsbm.32"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM198902093200606"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.2741%2F2853"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.6301011"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1124%2Fpr.54.2.203"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1538-7836.2005.01377.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1210%2Fer.2003-0025"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pain.2009.07.016"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pharmthera.2009.05.004"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M312090200"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M513138200"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.275.10.6819"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2003-04-1130"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002%2Fart.24300"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0706371"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2007-02-075440"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1159%2F000319751"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1742-1241.2008.01899.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1164%2Fajrccm%2F140.6.1712"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038%2Fsj.bjp.0701122"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.20071427"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1189%2Fjlb.0702351"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2007.11.025"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016%2FS0196-9781%2899%2900086-8"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.R500007200"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1038%2F42506"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/337378","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/337378","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:15:00Z","timestamp":1745421300000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000337378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":28,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2012,7,1]]},"published-print":{"date-parts":[[2012,7,3]]}},"URL":"https:\/\/doi.org\/10.1159\/000337378","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}