{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T11:00:41Z","timestamp":1776855641782,"version":"3.51.2"},"reference-count":38,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"published-print":{"date-parts":[[2012]]},"abstract":"<b><i>Background:<\/i><\/b> Among the five somatostatin receptors (sst<sub>1<\/sub>-sst<sub>5<\/sub>), the sst<sub>3<\/sub> receptor displays a distinct pharmacological profile. Like sst<sub>2<\/sub>, the sst<sub>3<\/sub> receptor efficiently internalizes radiolabeled somatostatin analogs. Unlike sst<sub>2<\/sub>, however, internalized sst<sub>3<\/sub> receptors are rapidly transferred to lysosomes for degradation. Apart from this, very little is known about the clinical relevance of the sst<sub>3<\/sub> receptor, which may in part be due to the lack of specific monoclonal sst<sub>3<\/sub> antibodies. <b><i>Methods:<\/i><\/b> Here, we have extensively characterized the novel rabbit monoclonal anti-human sst<sub>3<\/sub> antibody UMB-5 using transfected cells and receptor-expressing tissues. UMB-5 was then subjected to immunohistochemical staining of a series of 190 formalin-fixed, paraffin-embedded normal and neoplastic human tissues. <b><i>Results:<\/i><\/b> Specificity of UMB-5 was demonstrated by detection of a broad band migrating at a molecular weight of 70,000\u201385,000 in immunoblots from human pituitary. After enzymatic deglycosylation, the size of this band decreased to a molecular weight of 45,000. Tissue immunostaining was completely abolished by pre-adsorption of UMB-5 with its immunizing peptide. In addition, UMB-5 detected distinct cell populations in human tissues like pancreatic islands, anterior pituitary, adrenal cortex, adrenal medulla, and enteric ganglia, similar to that seen with a rabbit polyclonal antibody generated against a different carboxyl-terminal epitope of the sst<sub>3<\/sub> receptor. In a comparative immunohistochemical study, UMB-5 yielded predominant plasma membrane staining in the majority of pituitary adenomas, pheochromocytomas, and a subset of neuroendocrine tumors. The sst<sub>3<\/sub> receptor was also present in many glioblastomas, pancreatic, breast, cervix, and ovarian carcinomas. <b><i>Conclusion:<\/i><\/b> The rabbit monoclonal antibody UMB-5 may prove of great value in the identification of sst<sub>3<\/sub>-expressing tumors during routine histopathological examinations. Given its unique trafficking properties, these tumors may be potential candidates for sst<sub>3<\/sub>-directed receptor radiotherapy.<\/jats:p>","DOI":"10.1159\/000337659","type":"journal-article","created":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T09:54:49Z","timestamp":1331632489000},"page":"301-310","source":"Crossref","is-referenced-by-count":42,"title":["Reassessment of sst<sub>3<\/sub> Somatostatin Receptor Expression in Human Normal and Neoplastic Tissues Using the Novel Rabbit Monoclonal Antibody 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