{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:32:11Z","timestamp":1791181931502,"version":"4.1.0"},"reference-count":11,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T00:00:00Z","timestamp":1347408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T00:00:00Z","timestamp":1347408000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>It has recently been shown that approximately 80% of Merkel cell carcinomas harbor a novel polyomavirus named Merkel cell polyomavirus (MCPyV). MCPyV has been detected in human tissue samples. However, detailed distribution of MCPyV in non-neoplastic Japanese human tissues remains unclear. To address this, we used single or real-time quantitative polymerase chain reaction (PCR) for 41 autopsy cases. PCR revealed MCPyV-DNA in non-neoplastic samples: total, 29\/41 (71%); adult, 29\/39 (74%); fetus or infant, 0\/2; men, 24\/28 (86%); women, 5\/13 (38%); total human tissues, 66\/572 (12%); skin, 8\/15 (53%); adrenal gland, 9\/33 (27%), and other 16 organs (4\u201325%). This study first reported the presence of MCPyV-DNA in non-neoplastic tissues of thyroid gland, adrenal gland, spleen, bone marrow, stomach, gallbladder, pancreas, heart, and aorta. PCR revealed that viral load ranged from 0.00026 to 0.22 in all MCPyV-positive tissues compared with Merkel cell carcinoma samples. These detailed PCR data showed higher prevalence of MCPyV infection in Japanese men than women (p = 0.004) and broad distribution of MCPyV with low viral load in more non-neoplastic human tissues than in the previous reports. These data provide valuable insights for further studies of MCPyV infection and MCPyV-related diseases.<\/jats:p>","DOI":"10.1159\/000338620","type":"journal-article","created":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T08:06:39Z","timestamp":1347437199000},"page":"1-5","source":"Crossref","is-referenced-by-count":31,"title":["Detection of Merkel Cell Polyomavirus in the Human Tissues from 41 Japanese Autopsy Cases Using Polymerase Chain Reaction"],"prefix":"10.1159","volume":"56","author":[{"given":"Michiko","family":"Matsushita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Kuwamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Higaki-Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoji","family":"Yashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Horie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukisato","family":"Kitamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2012,9,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1126%2Fscience.1152586"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1371%2Fjournal.ppat.0030064"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3201%2Feid1503.081067"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3201%2Feid1503.081206"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002%2Fjmv.21608"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.3201%2Feid1604.091278"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.leukres.2011.01.032"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.humpath.2010.09.011"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2010-09-310557"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jcv.2010.10.015"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038%2Fjid.2011.115"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/338620","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/338620","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:21:17Z","timestamp":1745432477000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000338620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,12]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,11,1]]},"published-print":{"date-parts":[[2012,11,22]]}},"URL":"https:\/\/doi.org\/10.1159\/000338620","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"value":"0300-5526","type":"print"},{"value":"1423-0100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,12]]}}}