{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:25:39Z","timestamp":1761895539499,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2012]]},"abstract":"Occurrence of Philadelphia chromosome-negative myeloproliferative neoplasms (Ph<sup>\u2013<\/sup> MPN) and lymphoproliferative disorders, like B cell chronic lymphocytic leukaemia (B-CLL), in the same patient is rare. JAK2<sup>V617F<\/sup> mutation was recently introduced as a powerful diagnostic tool for Ph<sup>\u2013<\/sup> MPN. JAK2<sup>V617F<\/sup> mutation is not present in B-CLL. In 4 previously reported patients with JAK2<sup>V617F<\/sup>-positive Ph<sup>\u2013<\/sup> MPN and B-CLL there was no definitive proof of JAK2<sup>V617F<\/sup> mutation in B-CLL cells, although this was suggested in 1 patient. We present 2 patients with JAK2<sup>V617F<\/sup>-positive polycythaemia vera who subsequently developed a monoclonal B cell disorder. The granulocytes were separated from the mononuclear cells by centrifugation on density gradient. Using an ARIA-SORP sorter, the CD20+\/CD5+ B cells were separated from the CD20+\/CD5\u2013 B cells, T cells, NK cells and monocytes. On each of the fractions JAK2<sup>V617F<\/sup> mutation was analysed by allele-specific competitive blocker-PCR. In both patients JAK2<sup>V617F<\/sup> mutation was present in granulocytes confirming the clinical diagnosis of polycythaemia vera. We did not detect the JAK2<sup>V617F<\/sup> mutation in the CD20+\/CD5+ B cells but detected it in CD20+\/CD5\u2013 B cells, T and NK cells, indicating a lymphoid subdifferentiation of the JAK2<sup>V617F<\/sup> MPN clonality. JAK2<sup>V617F<\/sup> MPN and monoclonal B cell disorder can coexist but there is no evidence that the proliferative behaviour of these B cells is mediated through the JAK2<sup>V617F<\/sup> mutation.<\/jats:p>","DOI":"10.1159\/000338831","type":"journal-article","created":{"date-parts":[[2012,8,10]],"date-time":"2012-08-10T08:13:26Z","timestamp":1344586406000},"page":"183-186","source":"Crossref","is-referenced-by-count":13,"title":["No Evidence for JAK2<sup>V617F<\/sup> Mutation in Monoclonal B Cells in 2 Patients with Polycythaemia Vera and Concurrent Monoclonal B Cell Disorder"],"prefix":"10.1159","volume":"128","author":[{"given":"C.","family":"Stijnis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.G.M.","family":"Kroes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Balkassmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.W.A.","family":"Marijt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.P.","family":"van Rossum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bakker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.T.","family":"Vlasveld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,8,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMra063728"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1080%2F10428190600925772"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0601462103"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ccr.2011.06.029"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2141.2007.06497.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1182%2Fblood-2006-03-007146"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3324%2Fhaematol.10527"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI111840"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0142%2819921115%2970%3A10%3C2431%3A%3AAID-CNCR2820701008%3E3.0.CO%3B2-U"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.0902-4441.2006.t01-1-EJH2940.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002%2Fajh.20870"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159%2F000243721"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.leukres.2009.06.027"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002%2Fajh.22171"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1309%2F1U61JVXTLPPQ7YP1"}],"container-title":["Acta Haematologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/338831","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/338831","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/338831","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:23:08Z","timestamp":1745432588000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000338831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,9,1]]},"published-print":{"date-parts":[[2012,9,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000338831","archive":["Portico"],"relation":{},"ISSN":["0001-5792","1421-9662"],"issn-type":[{"type":"print","value":"0001-5792"},{"type":"electronic","value":"1421-9662"}],"subject":[],"published":{"date-parts":[[2012]]}}}