{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T13:51:22Z","timestamp":1773409882637,"version":"3.50.1"},"reference-count":33,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2012]]},"abstract":"<b><i>Background:<\/i><\/b> Extended-spectrum \u03b2-lactamase (ESBL)-producing organisms are spreading worldwide in hospital and community settings. <b><i>Methods:<\/i><\/b> A total of 328 unduplicated ESBL-producing Enterobacteriaceae isolated in 2008 and 2009 at the University Hospital of T\u00fcbingen were analysed retrospectively. <b><i>Results:<\/i><\/b><i>Escherichia coli<\/i> (n = 253) and <i>Klebsiella<\/i> spp. (n = 46) were the most frequent ESBL-producing species. The ESBL rates among <i>E. coli<\/i> and <i>Klebsiella<\/i> spp. increased from 3.8 and 2.1%, respectively, in 2008, to 5.2 and 2.4%, respectively, in 2009. Two <i>E. coli<\/i> and 3 <i>Klebsiella pneumoniae<\/i> ESBL producers were non-susceptible to ertapenem, most likely due to loss of porins. Antimicrobial susceptibility testing of selected, molecularly characterized ESBL producers revealed susceptibility to tigecycline among 97.9% (191\/195) of the <i>E. coli<\/i> and 78.8% (26\/33) of the <i>K. pneumoniae<\/i> isolates. PCR analysis and sequencing showed the presence of CTX-M-type enzymes in 91.3% of the <i>E. coli<\/i> and 87.9% of the <i>K. pneumoniae<\/i> isolates, whereby <i>bla<\/i><sub>CTX-M-15<\/sub> was the most frequent ESBL gene both in <i>E. coli<\/i> (50.0%) and <i>K. pneumoniae<\/i> (51.5%). Only 7 single cases of potential patient-to-patient transmissions of <i>E. coli<\/i> strains were observed. <b><i>Conclusions:<\/i><\/b> Our data suggest that the increase in ESBL-producing <i>E. coli<\/i> and <i>K. pneumoniae<\/i> isolates at our hospital is mainly caused by growing import of Enterobacteriaceae harbouring CTX-M-type ESBLs.<\/jats:p>","DOI":"10.1159\/000339488","type":"journal-article","created":{"date-parts":[[2012,7,25]],"date-time":"2012-07-25T08:18:20Z","timestamp":1343204300000},"page":"241-248","source":"Crossref","is-referenced-by-count":6,"title":["In vitro Activity of Tigecycline and Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing <b><i>Escherichia coli<\/i><\/b> and <b><i>Klebsiella pneumoniae<\/i><\/b> Isolates from a University Hospital in South-Western Germany"],"prefix":"10.1159","volume":"58","author":[{"given":"Matthias","family":"Wienke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Pfeifer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Weissgerber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Marschal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingo B.","family":"Autenrieth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Gr\u00f6bner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,7,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijantimicag.2009.11.003"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097%2FQCO.0b013e3283398dc1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkq463"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkp108"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1600-0463.2009.02465.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkp122"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.00009-11"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkn293"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000321018"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.00063-08"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159%2F000224657"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.00155-06"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.49.5.2137-2139.2005"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1099%2Fjmm.0.005850-0"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ijantimicag.2008.06.024"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.01988-06"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3201%2Feid1509.090567"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkm215"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1086%2F425018"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkr092"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.42.10.4769-4775.2004"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1469-0691.2007.01864.x"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.48.8.3203-3206.2004"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1159%2F000112415"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1159%2F000329406"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10096-008-0589-0"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.49.3.1017-1022.2005"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.00883-08"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1186%2F1471-2334-9-97"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1093%2Fjac%2Fdkp211"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1128%2FAAC.00068-06"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1086%2F500936"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1128%2FJCM.00447-06"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339488","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339488","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339488","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:29:33Z","timestamp":1745432973000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000339488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,8,1]]},"published-print":{"date-parts":[[2012,8,24]]}},"URL":"https:\/\/doi.org\/10.1159\/000339488","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"value":"0009-3157","type":"print"},{"value":"1421-9794","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}