{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:34:31Z","timestamp":1745469271508,"version":"3.40.4"},"reference-count":8,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T00:00:00Z","timestamp":1348531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T00:00:00Z","timestamp":1348531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2013]]},"abstract":"<b><i>Background:<\/i><\/b> Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play a role in the pathogenesis of asthma. MMP-9 increases in the sputum of asthmatic patients after bronchial challenge with common allergens. We sought to assess whether a high-molecular-weight occupational allergen was able to induce changes in MMP-9 as well as in other MMPs and TIMPs in subjects with occupational asthma. <b><i>Methods:<\/i><\/b> Ten patients underwent specific inhalation challenge (SIC) on 2 consecutive days. We monitored changes in lung function by measuring FEV<sub>1<\/sub> for 7 h. Induced sputum test was performed at 6 h after sham and flour challenge. The total and differential cell counts were analyzed. Levels of MMPs (specifically MMP-2, MMP-7, MMP-9 and MMP-13) were measured using Fluorokine\u00ae MultiAnalyte Profiling kits and a Luminex\u00ae Bioanalyzer, while levels of TIMP-1 and TIMP-2 were measured by ELISA. <b><i>Results:<\/i><\/b> Flour challenge increased the percentage of eosinophils in sputum samples. Asthmatic reactions induced by flour were associated with a significant increase in the sputum level of MMP-9 (p = 0.05), but not in the levels of MMP-2, MMP-7, MMP-13, TIMP-1 and TIMP-2. Sputum levels of MMP-9 measured after flour challenge were nearly significantly correlated (r = 0.67; p = 0.06) with the maximal fall in FEV<sub>1<\/sub> observed during the asthmatic reaction, but they did not correlate with the number of neutrophils (r = 0.18; p = 0.7) and eosinophils (r = 0.55; p = 0.2). <b><i>Conclusions:<\/i><\/b> This study showed that MMP-9 increases in sputum samples from sensitized occupational asthma patients after SIC with flour.<\/jats:p>","DOI":"10.1159\/000339737","type":"journal-article","created":{"date-parts":[[2012,9,25]],"date-time":"2012-09-25T17:51:38Z","timestamp":1348595498000},"page":"161-164","source":"Crossref","is-referenced-by-count":12,"title":["Matrix Metalloproteinase-9 Increases in the Sputum from Allergic Occupational Asthma Patients after Specific Inhalation Challenge"],"prefix":"10.1159","volume":"160","author":[{"given":"Roberto","family":"Castano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Miedinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Maghni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heberto","family":"Ghezzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole","family":"Trudeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucero","family":"Castellanos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathieu","family":"Wattiez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Vandenplas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Luc","family":"Malo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,9,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.coph.2004.12.005"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000077418"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1378%2Fchest.122.5.1553"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.97.10112612"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1378%2Fchest.122.5.1543"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2005.12.082"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1183%2F09031936.00019906"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1752-699X.2010.00195.x"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339737","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339737","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:32:14Z","timestamp":1745433134000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000339737"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,25]]},"references-count":8,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2013,1,1]]},"published-print":{"date-parts":[[2013,1,15]]}},"URL":"https:\/\/doi.org\/10.1159\/000339737","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"type":"print","value":"1018-2438"},{"type":"electronic","value":"1423-0097"}],"subject":[],"published":{"date-parts":[[2012,9,25]]}}}