{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T02:00:20Z","timestamp":1768269620757,"version":"3.49.0"},"reference-count":27,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2013]]},"abstract":"<b><i>Background:<\/i><\/b> Shellfish allergy is one of the major causes of life-threatening allergic reactions to food. The shrimp species <i>Pandalus borealis<\/i> is the commercially most important coldwater shrimp species, and its protein extract is commonly used in shrimp allergy diagnostics. However, the DNA sequence of its major allergen, tropomyosin, designated Pan b 1, was not previously described. Our aim was to identify the cDNA sequence of Pan b 1 and to generate a recombinant protein with similar structure and allergenicity as the natural protein. <b><i>Methods:<\/i><\/b><i>P. borealis<\/i> shrimps were caught in the Oslofjord (Norway). cDNA from Pan b 1 was generated, an N-terminal histidine tag was added, and the protein was expressed in <i>Escherichia coli<\/i>. The recombinant Pan b 1 was characterized by structural and IgE-binding studies and investigated further with basophil activation tests (BATs) and skin prick tests (SPTs) on Norwegian shrimp-allergic individuals. <b><i>Results:<\/i><\/b> The open reading frame encoded 284 amino acids that shared 97\u2013100% identity with other shrimp tropomyosins. Mass spectroscopy of natural Pan b 1 confirmed the protein\u2019s molecular mass and indicated the absence of posttranslational modifications. Circular dichroism spectroscopy revealed virtually identical spectra between recombinant and natural Pan b 1, which together with native PAGE and size exclusion chromatography results indicated a similar structure. Furthermore, immunoblot and ELISA studies as well as BATs and SPTs showed equivalent results of recombinant and natural Pan b 1. <b><i>Conclusion:<\/i><\/b> A recombinant tropomyosin from <i>P. borealis<\/i> was generated that can be used in diagnostics and further studies on tropomyosin allergenicity and specific immunotherapy.<\/jats:p>","DOI":"10.1159\/000339740","type":"journal-article","created":{"date-parts":[[2012,10,19]],"date-time":"2012-10-19T15:40:39Z","timestamp":1350661239000},"page":"221-232","source":"Crossref","is-referenced-by-count":11,"title":["Structural and Immunological Characterization of Recombinant Pan b 1, a Major Allergen of Northern Shrimp, <b><i>Pandalus borealis<\/i><\/b>"],"prefix":"10.1159","volume":"160","author":[{"given":"Heidi R.","family":"Myrset","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Barletta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"Di Felice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliann","family":"Egaas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maaike M.B.W.","family":"Dooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,10,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2010.03513.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2010.12.1098"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2010.03.015"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159%2F000236802"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2010.03.010"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jaci.2009.11.043"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1398-9995.2011.02663.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2891%2991497-H"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2F0968-0004%2879%2990003-3"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1271%2Fbbb.80751"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2F0091-6749%2894%2990156-2"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1021%2Fjf062798x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev-immunol-030409-101218"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1159%2F000109286"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2FS1081-1206%2810%2960881-2"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2F0076-6879%2882%2985023-4"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1186%2F1472-6750-10-8"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2222.2009.03426.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002%2Fbip.360300703"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002%2Fmnfr.200400015"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jmb.2009.07.039"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jprot.2010.10.010"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1074%2Fjbc.M707485200"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2FS0065-2881%2800%2938003-8"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1159%2F000048166"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.molimm.2011.06.216"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1750-3841.2009.01391.x"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339740","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339740","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:32:18Z","timestamp":1745433138000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000339740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,16]]},"references-count":27,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,2,1]]},"published-print":{"date-parts":[[2013,2,13]]}},"URL":"https:\/\/doi.org\/10.1159\/000339740","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,16]]}}}