{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T23:22:34Z","timestamp":1768000954020,"version":"3.49.0"},"reference-count":17,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Urol Int"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; This study aims to investigate whether mesothelial cells could function as seed cells to construct tissue-engineered peritoneum-like tissue for urethral reconstruction in a rabbit model. &lt;b&gt;&lt;i&gt;Materials and Methods: &lt;\/i&gt;&lt;\/b&gt;Bladder acellular matrices were prepared and trimmed to 1.5 \u00d7 1 cm. Nine male rabbits underwent omentum biopsy and autologous mesothelial cells were isolated. After in vitro expansion, the cells were seeded onto the matrices and incubated for 7 days. In 18 rabbits, a pendulous urethral segment 1.5 cm long was totally excised and urethroplasty was performed with tubularized matrices seeded with cells in 9 animals and without cells in 9 as controls. Urethrography was performed at 1, 2 and 6 months postoperatively. Meanwhile, the neourethras were harvested and analyzed grossly and histologically. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Histological analysis of the cell-seeded grafts revealed a loose collagen matrix covered with a single layer of mesothelim. Serial urethrography indicated a wide urethral caliber without stricture formation in animals implanted with cell-seeded matrices, while all animals of the control group developed stricture. Histological analysis of the implanted cell-seeded matrices demonstrated a normal urethral architecture by 1 month, composed of multilayers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. By 6 months, the neourethra could be hardly distinguished from native urethra both grossly and histologically. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; Autologous mesothelial cells could be successfully used as seed cells for tubularized urethral reconstruction in male rabbits.<\/jats:p>","DOI":"10.1159\/000339745","type":"journal-article","created":{"date-parts":[[2012,7,11]],"date-time":"2012-07-11T09:10:14Z","timestamp":1341997814000},"page":"358-364","source":"Crossref","is-referenced-by-count":24,"title":["Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model"],"prefix":"10.1159","volume":"89","author":[{"given":"Gang-Li","family":"Gu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Jie","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Hua","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Jian","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,7,11]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.juro.2006.03.097"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1442-2042.2000.00210.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.juro.2007.07.034"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00345-008-0316-6"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ju.0000089776.52568.9e"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097%2F00005392-200210020-00032"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1464-410X.2006.06691.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.juro.2008.06.013"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.biocel.2003.11.002"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1523-1755.2003.00772.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cardiores.2005.05.022"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0022-3468%2896%2990002-0"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1097%2F00005392-200102000-00074"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.juro.2007.05.062"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1097%2F01.ju.0000101881.80100.ab"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00345-009-0447-4"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002%2Fterm.66"}],"container-title":["Urologia Internationalis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339745","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/339745","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:32:23Z","timestamp":1745433143000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000339745"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":17,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2012,10,1]]},"published-print":{"date-parts":[[2012,10,24]]}},"URL":"https:\/\/doi.org\/10.1159\/000339745","archive":["Portico"],"relation":{},"ISSN":["0042-1138","1423-0399"],"issn-type":[{"value":"0042-1138","type":"print"},{"value":"1423-0399","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}