{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T10:54:49Z","timestamp":1753181689330,"version":"3.40.4"},"reference-count":12,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2012,10,5]],"date-time":"2012-10-05T00:00:00Z","timestamp":1349395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2012,10,5]],"date-time":"2012-10-05T00:00:00Z","timestamp":1349395200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; Despite the endemicity of hepatitis A virus (HAV) in Nigeria, genetic information on the HAV genotypes\/subgenotypes circulating in the country remains unknown. The objective of this study was to investigate HAV strains using molecular epidemiological and genetic analyses among apparently healthy adult Nigerian subjects. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Testing for HAV-RNA was performed on 114 serum samples by the reverse transcription-polymerase chain reaction and sequenced with primers encompassing the VP1\/P2A junction. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Twelve serum samples tested were found to be HAV-RNA positive. Phylogenetic analysis revealed that all 12 HAV isolates were classified as subgenotype IA exhibiting 98.3% nucleotide identity. Interestingly, the Nigerian HAV\/IA subgenotype consisted of two distinct genomic sublineages with a unique majority (n = 11) corresponding to strains endemic in Cameroon and the other (n = 1) shows a probable link with European sequences. Predicted conserved amino acid sequences and the few deduced substitution in the VP1\/P2A junction might play a role in the development of a novel Nigerian-Cameroon sublineage within the HAV\/IA subgenotype and might explain the stability of HAV\/IA in this subregion. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; This study reveals the development of a new HAV\/IA sublineage in the Nigerian-Cameroon subregion. The presence of a single subgenotype indicates that this HAV strain has been predominantly circulating in Nigeria.<\/jats:p>","DOI":"10.1159\/000341612","type":"journal-article","created":{"date-parts":[[2012,10,5]],"date-time":"2012-10-05T07:13:48Z","timestamp":1349421228000},"page":"22-26","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Characterization of Hepatitis A Virus Isolates from Nigeria"],"prefix":"10.1159","volume":"56","author":[{"given":"Joseph C.","family":"Forbi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathew D.","family":"Esona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon M.","family":"Agwale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,10,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1440-1746.2000.02141.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1128%2FCMR.19.1.63-79.2006"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1128%2FCMR.14.1.38-58.2001"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1071%2FSH10162"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1001%2Farchinternmed.2010.401"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Fmolbev%2Fmsr121"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007%2FBF01731581"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1099%2F0022-1317-73-6-1365"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.vaccine.2009.10.136"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128%2FJVI.74.22.10498-10507.2000"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002%2Fjmv.22086"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1086%2F315701"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/341612","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/341612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:39:37Z","timestamp":1745433577000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000341612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,5]]},"references-count":12,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,11,1]]},"published-print":{"date-parts":[[2012,11,22]]}},"URL":"https:\/\/doi.org\/10.1159\/000341612","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"type":"print","value":"0300-5526"},{"type":"electronic","value":"1423-0100"}],"subject":[],"published":{"date-parts":[[2012,10,5]]}}}