{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:15:36Z","timestamp":1778667336668,"version":"3.51.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T00:00:00Z","timestamp":1345075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"},{"start":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T00:00:00Z","timestamp":1345075200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med Princ Pract"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; To study the potential of chemically modified tetracycline-3 (COL-3), a potent matrix metalloproteinase (MMP) inhibitor, to protect against the development of paclitaxel-induced painful neuropathy and its immunomodulatory effects. &lt;b&gt;&lt;i&gt;Materials and Methods:&lt;\/i&gt;&lt;\/b&gt; The reaction latency to thermal stimuli (hot plate test) of female BALB\/c mice was recorded before and after treatment with paclitaxel (2 mg\/kg i.p.), paclitaxel plus COL-3 (4, 20 or 40 mg\/kg p.o.) or their vehicles for 5 consecutive days. Gene transcripts of CD11b (marker for microglia), 5 cytokines (IFN-\u03b3, IL-1\u03b2, IL-6, IL-10 and TNF-\u03b1) and 3 chemokines (CCL2, CXCL10 and CX3CL1) were quantified by real-time PCR in the brains, spinal cords and spleens of mice sacrificed on day 7 after treatment. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Treatment with paclitaxel reduced the reaction latency time to thermal stimuli (thermal hyperalgesia) for 4 weeks, with maximum effect on days 7 and 10. The coadministration of paclitaxel with COL-3 40 mg\/kg, but not lower doses, prevented the development of paclitaxel-induced thermal hyperalgesia. Treatment with paclitaxel alone or coadministration with COL-3 increased CD11b transcript levels in the brain but not in the spinal cord. Treatment with paclitaxel reduced IL-6 transcript levels in the spinal cord but did not alter the transcript levels of other cytokines or chemokines in the brain, spinal cord or spleen. The coadministration of COL-3 with paclitaxel significantly increased the transcript levels of IL-6 in the spleen and decreased CX3CL1 transcripts in the brain in comparison to treatment with paclitaxel alone. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Our results indicate that the MMP inhibitor COL-3 protected against paclitaxel-induced thermal hyperalgesia and, thus, could be useful in the prevention of chemotherapy-induced painful neuropathy.<\/jats:p>","DOI":"10.1159\/000341710","type":"journal-article","created":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T09:12:49Z","timestamp":1345108369000},"page":"35-41","source":"Crossref","is-referenced-by-count":27,"title":["Matrix Metalloproteinase Inhibitor COL-3 Prevents the Development of Paclitaxel-Induced Hyperalgesia in Mice"],"prefix":"10.1159","volume":"22","author":[{"given":"Subramanian S.","family":"Parvathy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willias","family":"Masocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2012,8,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159%2F000086183"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1526-4637.2001.002001008.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pain.2004.01.021"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00280-008-0689-7"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.pain.2007.10.012"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093%2Ffampra%2Fcmh115"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.brainres.2007.06.066"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jpain.2011.12.002"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.ejphar.2012.02.008"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.bbi.2006.10.012"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.neuroscience.2011.06.085"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1349-7006.2008.00877.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016%2FS8756-3282%2897%2900221-4"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1080%2F07853890500300386"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2F0304-3959%2883%2990201-4"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jneuroim.2009.06.022"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1006%2Fmeth.2001.1262"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1186%2F1744-8069-6-76"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.drup.2004.04.002"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.phrs.2011.05.011"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.brainresrev.2011.01.002"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jneuroim.2008.04.011"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.phrs.2010.11.003"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.phrs.2010.10.008"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007%2Fs10637-006-9031-6"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1200%2FJCO.2005.04.2614"}],"container-title":["Medical Principles and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/341710","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/341710","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/341710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:40:42Z","timestamp":1745433642000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000341710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,16]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2012,12,1]]},"published-print":{"date-parts":[[2012,12,14]]}},"URL":"https:\/\/doi.org\/10.1159\/000341710","archive":["Portico"],"relation":{},"ISSN":["1011-7571","1423-0151"],"issn-type":[{"value":"1011-7571","type":"print"},{"value":"1423-0151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,16]]}}}