{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:48:12Z","timestamp":1770972492483,"version":"3.50.1"},"reference-count":16,"publisher":"S. Karger AG","issue":"Suppl. 1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2013]]},"abstract":"<b><i>Objectives:<\/i><\/b> Hepatocellular carcinoma (HCC) is one of the common cancers worldwide. Accurate diagnosis of tumor progression is critical for the appropriate management of HCC. Here, we established a sensitive assay to detect and quantify tumor-derived DNA in the serum of HCC patients. <b><i>Methods:<\/i><\/b> Aberrant methylation of the <i>APC<\/i> gene was quantified in 23 HCC patients and 8 healthy volunteers using 100 \u00b5l of serum. For sensitive detection and accurate quantification of tumor DNA, we combined seminested polymerase chain reaction (PCR) with TaqMan PCR, which could amplify the <i>APC <\/i>gene regardless of the methylation status and detect the methylated and unmethylated sequences separately. The ratio of methylated to unmethylated sequences was quantified. <b><i>Results:<\/i><\/b> The methylated <i>APC<\/i> gene was detected in all HCC patients examined, but no healthy volunteers showed amplification of methylated sequences in serum. HCC patients with portal vein thrombosis showed a significantly higher methylated to unmethylated <i>APC<\/i> gene ratio in serum than those without portal vein thrombosis (p = 0.0029). <b><i>Conclusions:<\/i><\/b> Considering the strong association between the ratio of the methylated to unmethylated <i>APC<\/i> sequences in serum and the presence of portal vein thrombosis, methylation status of <i>APC<\/i> sequences could be a promising marker for improving HCC management.<\/jats:p>","DOI":"10.1159\/000345895","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T09:37:10Z","timestamp":1361353030000},"page":"82-87","source":"Crossref","is-referenced-by-count":9,"title":["Quantification of Tumor DNA in Serum and Vascular Invasion of Human Hepatocellular Carcinoma"],"prefix":"10.1159","volume":"84","author":[{"given":"Naoshi","family":"Nishida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadaaki","family":"Arizumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Takita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Kitai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norihisa","family":"Yada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Hagiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunori","family":"Minami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuomi","family":"Ueshima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiharu","family":"Sakurai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Ida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Kudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,2,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159%2F000339016"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000342403"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159%2F000339019"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJMoa0708857"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002%2F1097-0142%2820010201%2991%3A3%3C561%3A%3AAID-CNCR1035%3E3.0.CO%3B2-N"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1053%2Fj.gastro.2009.10.031"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.2174%2F138920211795564359"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038%2Fnm.1789"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002%2Fhep.25706"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002%2Fhep.22110"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1158%2F0008-5472.CAN-06-3464"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038%2Fng.2291"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1158%2F1078-0432.CCR-06-1900"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1158%2F1078-0432.CCR-11-2175"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.cca.2010.09.028"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1093%2Fjnci%2F92.22.1805"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/345895","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/345895","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/345895","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:40:23Z","timestamp":1745433623000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000345895"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":16,"journal-issue":{"issue":"Suppl. 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