{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T18:06:40Z","timestamp":1758478000687,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2013]]},"abstract":"<b><i>Background:<\/i><\/b> The accrual of human tissues from autopsies for diagnostic and translational research has decreased significantly over the last decades. <b><i>Objectives:<\/i><\/b> The objective of this study was to evaluate our experience with lung biopsy through a minithoracotomy as an alternative for obtaining postmortem tissue when full autopsy is refused in congenital diaphragmatic hernia (CDH) patients. <b><i>Methods:<\/i><\/b> Within 2 h of death we routinely asked parents for permission to perform an autopsy. Starting in 2001, parents who refused autopsy were asked permission for a postmortem lung biopsy. Pathology autopsy and biopsy reports were compared to clinical records. <b><i>Results:<\/i><\/b> Between 2001 and 2009, 46 patients died from CDH. Permission for autopsy was granted in 5 patients (11%). Of the remaining 41 patients, the parents of 15 (33%) agreed to postmortem lung biopsy. In all cases, additional findings were reported from the autopsy or biopsy, without changing the originally reported cause of death<i>.<\/i> In 1 case, we isolated fibroblasts from the lung biopsy using standardized cell culture techniques. Parents were able to take their child home with a minimal delay following biopsy. <b><i>Conclusions:<\/i><\/b> Parents refusing a full autopsy frequently agree to postmortem organ biopsy. This approach should therefore be considered as a valuable alternative, when permission for full autopsy is declined, for obtaining human tissues for both diagnostic and research purposes and is potentially applicable to other anomalies.<\/jats:p>","DOI":"10.1159\/000345921","type":"journal-article","created":{"date-parts":[[2013,1,14]],"date-time":"2013-01-14T07:53:03Z","timestamp":1358149983000},"page":"213-217","source":"Crossref","is-referenced-by-count":4,"title":["Postmortem Biopsy to Obtain Lung Tissue in Congenital Diaphragmatic Hernia"],"prefix":"10.1159","volume":"103","author":[{"given":"R.B.","family":"Van Loenhout","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.R.","family":"De Krijger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.P.","family":"Van de Ven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.W.J.M.","family":"Van der Horst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.W.J.E.","family":"Beurskens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Tibboel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Keijzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,1,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1136%2Fbmj.324.7340.761"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159%2F000329094"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1136%2Ffn.85.1.F4"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2F0002-9343%2886%2990822-3"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.54.11.820"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1136%2Fadc.84.6.455"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1002%2Fbjs.1800791218"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM198304283081704"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159%2F000322844"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1136%2Fjme.19.3.164"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1203%2F01.pdr.0000246245.05530.02"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2FS0002-9440%2810%2963848-5"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1056%2FNEJM197808172990704"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1080%2F00313029500169532"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00428-007-0548-7"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/345921","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/345921","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:40:54Z","timestamp":1745433654000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000345921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2013,3,1]]},"published-print":{"date-parts":[[2013,3,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000345921","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5124382","asserted-by":"subject"}]},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2013]]}}}