{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:35:29Z","timestamp":1745469329523,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T00:00:00Z","timestamp":1363305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T00:00:00Z","timestamp":1363305600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Physiol"],"abstract":"<b><i>Background\/Aims:<\/i><\/b> Since furosemide (FS) inhibits active Na<sup>+<\/sup> reabsorption by medullary thick ascending limb (mTAL) in the renal outer medulla, it may decrease its work during periods of low O<sub>2<\/sub> supply to deep in the renal outer medulla. This study was designed to demonstrate that there may be a dose of FS would reduce its metabolic work while preventing the excessive loss of magnesium (Mg<sup>2+<\/sup>). Mg<sup>2+<\/sup> is important because the ATP needed to perform work must have bound Mg<sup>2+<\/sup> to it. <b><i>Methods:<\/i><\/b> Rats were injected intraperitoneally with a range of doses of FS. The measured outcomes were urine flow rate and parameters of functions of the mTAL (i.e. urine and renal papillary osmolality and urinary excretion of Na<sup>+<\/sup>, Cl<sup>-<\/sup>, K<sup>+<\/sup> and Mg<sup>2+<\/sup>, and concentrations of Mg<sup>2+<\/sup> in serum). <b><i>Results:<\/i><\/b> The urine flow rate increased significantly starting at 2.4 mg FS\/kg. The renal papillary osmolality decreased at \u22650.4 mg FS\/kg, and the large detectable natriuresis started at 1.6 mg FS\/kg. At this latter dose, the urinary excretion of Mg<sup>2+<\/sup> rose significantly. <b><i>Conclusion:<\/i><\/b> In rats, the non-natriuretic dose of FS may reduce the work of the mTAL. The earliest indicator of reduced work in the mTAL appears to be a decrease in urine osmolality rather than a rise in urine flow rate. Higher doses of FS should be avoided, as they induce high rates of Mg<sup>2+<\/sup> excretion, which can deplete the body of this essential electrolyte.<\/jats:p>","DOI":"10.1159\/000346741","type":"journal-article","created":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T14:39:09Z","timestamp":1363358349000},"page":"7-12","source":"Crossref","is-referenced-by-count":1,"title":["Non-Natriuretic Doses of Furosemide: Potential Use for Decreasing the Workload of the Renal Outer Medulla with Minimal Magnesium Wasting in the Rat"],"prefix":"10.1159","volume":"122","author":[{"given":"Namhee","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surinder","family":"Cheema-Dhadli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory M.T.","family":"Hare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chee Kiong","family":"Chong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell L.","family":"Halperin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamel S.","family":"Kamel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. David","family":"Mazer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,3,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2006.04472.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-4431.2007.00267.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1139\/y93-017"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.101576"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7152(93)90015-B"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015951710204"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1988.00380020225030"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000079042.13465.1A"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199503093321006"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199605303342207"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.2463\/mrms.9.187"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1997.443"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136\/heart.89.4.411"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400958200"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2007010056"}],"container-title":["Nephron Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/346741","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/346741","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:49:16Z","timestamp":1745434156000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000346741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,15]]},"references-count":15,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2013,4,1]]},"published-print":{"date-parts":[[2013,4,12]]}},"URL":"https:\/\/doi.org\/10.1159\/000346741","archive":["Portico"],"relation":{},"ISSN":["1660-2137"],"issn-type":[{"type":"electronic","value":"1660-2137"}],"subject":[],"published":{"date-parts":[[2013,3,15]]}}}