{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T23:36:49Z","timestamp":1790465809253,"version":"4.1.0"},"reference-count":25,"publisher":"S. Karger AG","issue":"4-5","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Blood Press Res"],"published-print":{"date-parts":[[2013]]},"abstract":"<b><i>Background: <\/i><\/b>This work aims to explore the role of Th17 and IL-17 signaling in the pathogenesis of primary nephrotic syndrome (PNS) in children and podocyte injury, children with PNS were divided into minimal change nephrotic syndrome (MCNS) and non-minimal change nephrotic syndrome [NMCNS, including mesangial proliferative glomerulonephritis (MsPGN) and focal segmental glomerulosclerosis (FSGS)]. <b><i>Methods: <\/i><\/b>Flow cytometry (FCM) was used to observe the circulating frequency of Th17 cells and the apoptosis of podocytes by annexinV-FITC\/PI. Serum IL-1\u03b2 and IL-6 levels were measured using enzyme-linked immunosorbent assay. The Fas and FasL expressions in podocytes were examined by FCM analysis using a direct immunofluorescence method. Reverse transcription polymerase chain reaction was applied to measure the mRNA expressions of RORc, IL-23p19, Nephrin, WT1, Synaptopodin, Podocalyxin, Fas, and FasL. The IL-17 and IL-1\u03b2 expression in renal biopsy tissue was detected by immunohistochemistry. The expressions of WT1, Caspase 8, and Caspase 3 in podocyte cell culture were also measured using immunocytochemistry. <b><i>Results: <\/i><\/b>Circulating frequencies of Th17 cells, mRNA levels of RORc and IL-23p19, and serum levels of IL-6 and IL-1\u03b2 were higher in the MCNS and NMCNS groups than in the control group (all <i>P<\/i> < 0.05), and were higher in the NMCNS group than in the MCNS group (all <i>P<\/i> < 0.05). The expressions of IL-17 and IL-1\u03b2 in renal biopsy tissue were higher in the MCNS, MsPGN, and FSGS groups than in the control group (all <i>P<\/i> < 0.05). Recombinant murine IL-17 (rmIL-17) had no effect on the expressions of Nephrin, Synaptopodin, and WT1 of mouse podocytes, but caused an decrease in the expression of podocalyxin as well as promoted apoptosis in a dose- and time-dependent fashion. Moreover, rmIL-17 increased the expression of Fas, Casepase-8, and Casepase-3, but had no effect on that of FasL. <b><i>Conclusion<\/i><\/b><b><i>: <\/i><\/b>Th17\/IL-17 may contribute to the pathogenesis of PNS by decreasing the podocalyxin level and inducing podocyte apoptosis.<\/jats:p>","DOI":"10.1159\/000350161","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T08:51:22Z","timestamp":1381222282000},"page":"332-345","source":"Crossref","is-referenced-by-count":59,"title":["The Role of Th17\/IL-17 in the Pathogenesis of Primary Nephrotic Syndrome in Children"],"prefix":"10.1159","volume":"37","author":[{"given":"Li","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuicui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2013,9,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2002.kid561.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000045609"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(74)91880-7"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1053\/ajkd.2002.32769"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-009-1194-x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00496.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002291"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00365.x"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.035"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/ni1497"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038\/ni1496"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/nri2295"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009040379"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.14.3990"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00472.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.2174\/157339908783502370"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004070564"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/17.suppl_9.11"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050398"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2002.00269.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.12.1965"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.9.3219"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.060003957.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.03039.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.14.10413"}],"container-title":["Kidney and Blood Pressure Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/350161","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/350161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/350161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:15:35Z","timestamp":1745432135000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000350161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":25,"journal-issue":{"issue":"4-5","published-online":{"date-parts":[[2013,11,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000350161","archive":["Portico"],"relation":{},"ISSN":["1420-4096","1423-0143"],"issn-type":[{"value":"1420-4096","type":"print"},{"value":"1423-0143","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}