{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:10:35Z","timestamp":1764655835984,"version":"3.40.4"},"reference-count":23,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; The aim of this study was to evaluate whether the immunocytochemical expression of cell proliferation markers, such as minichromosome maintenance protein 7 (MCM 7), geminin, topoisomerase II alpha (topo II\u03b1) and Ki-67, which are different types of cell proliferation markers, could be useful for their differential diagnosis in reactive mesothelial cells and malignant mesothelioma cells obtained from body cavity fluids. &lt;b&gt;&lt;i&gt;Study Design:&lt;\/i&gt;&lt;\/b&gt; Samples diagnosed and later histologically confirmed as reactive mesothelial cells (39 cases) or malignant mesothelioma (32 cases) in body cavity fluids were examined. Immunocytochemical staining of MCM 7, geminin, topo II\u03b1 and Ki-67 was performed with the immunoperoxidase polymer method. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Labeling indices (LIs) of MCM 7 (cutoff value 20.0%; sensitivity 100%; specificity 100%), geminin (cutoff value 4.5%; sensitivity 88.0%; specificity 70.0%), topo II\u03b1 (cutoff value 11.0%; sensitivity 88.0%; specificity 92.0%) and Ki-67 (cutoff value 15.3%; sensitivity 78.0%; specificity 79.0%) of malignant mesothelioma cells were significantly higher than those of reactive mesothelial cells. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; LIs of MCM 7, geminin and topo II\u03b1 can be reliable tools for the differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells.<\/jats:p>","DOI":"10.1159\/000350262","type":"journal-article","created":{"date-parts":[[2013,7,12]],"date-time":"2013-07-12T06:55:23Z","timestamp":1373612123000},"page":"384-390","source":"Crossref","is-referenced-by-count":13,"title":["Differential Diagnosis of Reactive Mesothelial Cells and Malignant Mesothelioma Cells Using the Cell Proliferation Markers Minichromosome Maintenance Protein 7, Geminin, Topoisomerase II Alpha and Ki-67"],"prefix":"10.1159","volume":"57","author":[{"given":"Fumikazu","family":"Kimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Okayasu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokuni","family":"Kakinuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukitoshi","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadahito","family":"Kuwao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Saegusa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,7,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2005.00714.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050152"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000329817"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21303"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0214-OA"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21190"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81209-X"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5500.2309"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5500.2271"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22474"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1002\/path.1625"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206987"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2006.00178.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.134"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-010-0558-z"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20071"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500397102"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/path.1708"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.4.1086"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20938"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1159\/000331291"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199802)18:2<141::AID-DC11>3.0.CO;2-L"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20747"}],"container-title":["Acta 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