{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:34:24Z","timestamp":1745469264259,"version":"3.40.4"},"reference-count":18,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2013]]},"abstract":"<b><i>Objectives:<\/i><\/b> The aim of this study was to assess the diagnostic usefulness of vascular endothelial growth factor (VEGF) and endostatin mRNA in cervical specimens of patients with cervical dysplasia and carcinoma. <b><i>Study Design:<\/i><\/b> Transcription levels of VEGF and endostatin were detected by RT-PCR in cervical liquid-based preparation specimens and compared with cytological assessments. <b><i>Results:<\/i><\/b> VEGF as well as endostatin mRNA expression was significantly associated with either cytological or histological diagnosis (p < 0.05). VEGF mRNA and endostatin mRNA were significantly more likely to be expressed in squamous cell carcinomas (SCC) than in cervical intraepithelial neoplasia (CIN) III (p < 0.01 and p < 0.05), and obviously also more likely to be expressed in CINII than in CINI and in CINIII than in CINII (p < 0.05). Eleven inflammation lesions gave positive results by cytology but negative results by RT-PCR for VEGF and endostatin mRNA. Twenty-four SCC lesions gave false-negative or precancerous lesion results by cytology but positive results by RT-PCR for VEGF and\/or endostatin mRNA expression. <b><i>Conclusion:<\/i><\/b> Transcription levels of VEGF and endostatin by RT-PCR may be an adjunct to cytology screening for early detection of cervical carcinomas and may determine the progressive potentiality of individual lesions, especially in high-risk patients.<\/jats:p>","DOI":"10.1159\/000351776","type":"journal-article","created":{"date-parts":[[2013,9,9]],"date-time":"2013-09-09T08:41:12Z","timestamp":1378716072000},"page":"522-527","source":"Crossref","is-referenced-by-count":2,"title":["Transcription Expression and Clinical Significance of mRNA of Vascular Endothelial Growth Factor and Endostatin in Liquid-Based Preparation Specimens from Patients with Cervical Dysplasia and Carcinoma"],"prefix":"10.1159","volume":"57","author":[{"given":"Ming-Zhe","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Min","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-Ping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-Jun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,9,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-31-1"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/35025220"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.06.081"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/87.16.1237"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(98)00410-9"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(02)02970-X"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207442"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.05.030"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00262-08"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1989.03420050087046"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.45.5.305"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20926"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2099.101837"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006208303075"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2011.02008.x"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2007.01.051"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0b013e3181bb8034"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1319"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/351776","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/351776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:30:05Z","timestamp":1745433005000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000351776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":18,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,10,1]]},"published-print":{"date-parts":[[2013,10,11]]}},"URL":"https:\/\/doi.org\/10.1159\/000351776","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"type":"print","value":"0001-5547"},{"type":"electronic","value":"1938-2650"}],"subject":[],"published":{"date-parts":[[2013]]}}}