{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:53:20Z","timestamp":1761663200280,"version":"3.40.4"},"reference-count":18,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objectives:&lt;\/i&gt;&lt;\/b&gt; We compared the efficacy of automated and manual human papilloma virus (HPV) in situ hybridization (ISH) and p16 immunohistochemical staining (IHC) in fine needle aspiration (FNA) of metastatic oropharyngeal carcinoma. &lt;b&gt;&lt;i&gt;Study Design:&lt;\/i&gt;&lt;\/b&gt; A total of 41 FNA cell blocks (CB) were evaluated. HPV ISH was interpreted as positive if a minimum of one tumor cell showed punctate dot-like nuclear positivity. p16 was interpreted as positive if \u226570% of tumor cells showed brown nuclear and cytoplasmic staining. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Thirty of 41 CB (73%) were positive by automated HPV ISH, 25 of 41 CB (60%) with manual HPV ISH. Eighteen of 41 CB (43%) were positive for p16 IHC. Twelve of 41 CB (29%) with automated HPV ISH and 2 of 41 CB (4%) with the manual method were positive at 10\u00d7 magnification. Three of 41 CB (7%) with automated HPV ISH and 14 of 41 CB (34%) with the manual method were positive at 20\u00d7 magnification. Fifteen of 41 CB (36%) with automated HPV ISH and 9 of 41 CB (21%) with the manual method were positive at 40-60\u00d7 magnification. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Automated HPV ISH plays a more significant role in determining the HPV status in CB. However, the failure to use high magnification in the evaluation can give false-negative results.<\/jats:p>","DOI":"10.1159\/000353225","type":"journal-article","created":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T12:37:37Z","timestamp":1380631057000},"page":"633-640","source":"Crossref","is-referenced-by-count":7,"title":["Automated and Manual Human Papilloma Virus in situ Hybridization and p16 Immunohistochemistry: Comparison in Metastatic Oropharyngeal Carcinoma"],"prefix":"10.1159","volume":"57","author":[{"given":"Nazneen","family":"Fatima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane","family":"Lawson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momin T.","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,10,1]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100128907"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(61)90679-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0278-2391(89)90117-1"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-200105000-00009"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1313"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.9.709"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1186\/1758-3284-2-6"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21293"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690229"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/sj\/onc\/1205214"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63871-0"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1177\/002215540305101006"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-198201000-00006"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0278-2391(89)90117-1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200010000-00012"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21027"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1690"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/hed.20796"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/353225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/353225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:34:19Z","timestamp":1745433259000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000353225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":18,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,12,1]]},"published-print":{"date-parts":[[2013,11,29]]}},"URL":"https:\/\/doi.org\/10.1159\/000353225","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"type":"print","value":"0001-5547"},{"type":"electronic","value":"1938-2650"}],"subject":[],"published":{"date-parts":[[2013]]}}}