{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T14:25:37Z","timestamp":1774103137709,"version":"3.50.1"},"reference-count":21,"publisher":"S. Karger AG","issue":"3-4","license":[{"start":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T00:00:00Z","timestamp":1375747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T00:00:00Z","timestamp":1375747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Clin Pract"],"abstract":"<b><i>Background\/Aims:<\/i><\/b> S100A12 induces vascular inflammation contributing to the development of atherosclerosis. Serum S100A12 concentration is shown to be elevated in patients with chronic kidney disease (CKD), however the reason remains unclear. <b><i>Methods:<\/i><\/b> Transcriptional levels of S100A12 and RAGE (receptor for advanced glycation end products) were measured in peripheral leukocytes by quantitative real-time RT-PCR. Subjects were 40 patients with CKD stage 4-5, 20 of whom were affected with cardiovascular disease (CVD), and 20 healthy subjects. Serum concentrations of S100A12 and soluble RAGE were measured using enzyme-linked immunosorbent assay. <b><i>Results:<\/i><\/b> The serum concentration of S100A12 was significantly higher in CKD patients than in healthy subjects<b> <\/b>(78.5 \u00b1 70.5 vs. 23.7 \u00b1 19.2 ng\/ml, p = 0.0035), but that of soluble RAGE was not.<b> <\/b>The relative quantity of<b> <\/b>S100A12 mRNA was significantly greater in leukocytes from CKD patients than in those from healthy subjects [mean (95% confidence interval of the mean): 3.1 (2.2-3.9) vs. 1.2 (0.8-1.7), p = 0.0001], however that of RAGE mRNA was not. The serum concentration of S100A12 was significantly correlated with the relative quantity of S100A12 mRNA among uremic CKD patients (r<sup>2<\/sup> = 0.656, p < 0.0001). Both the serum concentration and gene expression of S100A12 were significantly higher in patients who had CVD than in those who did not. <b><i>Conclusion:<\/i><\/b> Excessive expression of<b> <\/b>the<b> <\/b>S100A12 gene in uremic leukocytes is relevant to its increased serum concentration, particularly in those affected with CVD.<\/jats:p>","DOI":"10.1159\/000353808","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T08:47:21Z","timestamp":1375778841000},"page":"202-208","source":"Crossref","is-referenced-by-count":8,"title":["S100A12 Gene Expression Is Increased in Peripheral Leukocytes in Chronic Kidney Disease Stage 4-5 Patients with Cardiovascular Disease"],"prefix":"10.1159","volume":"123","author":[{"given":"Masaki","family":"Hara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Ando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taku","family":"Morito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohiko","family":"Nokiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Iwasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Tsuchiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosaku","family":"Nitta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,8,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(01)00046-2"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80801-6"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000131783.74034.97"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.209486"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-032223"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.08310910"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1159\/000148646"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.03360410"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-9987.2007.00537.x"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2011.07.115"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2008.12.034"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-11-31"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409782200"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2003.08.021"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900373"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-156-8-201204170-00004"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2010.58"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2005.12.028"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.419"}],"container-title":["Nephron Clinical Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/353808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/353808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:39:34Z","timestamp":1745433574000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000353808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,6]]},"references-count":21,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2013,11,1]]},"published-print":{"date-parts":[[2013,11,21]]}},"URL":"https:\/\/doi.org\/10.1159\/000353808","archive":["Portico"],"relation":{},"ISSN":["1660-2110"],"issn-type":[{"value":"1660-2110","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,6]]}}}