{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:35:06Z","timestamp":1745469306389,"version":"3.40.4"},"reference-count":15,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; Histamine remains the main mediator of allergic conjunctivitis and induces interleukin (IL)-6 and IL-8 production in human conjunctival epithelial cells (HCEC). The purpose of the present study was to determine whether histamine induced IL-6 and IL-8 expression in HCEC, and to describe the relationship between human calcium-activated chloride channel (hCLCA) 1 activity and IL-6 and IL-8 expression. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; HCEC were isolated from human conjunctiva and cultured at an air-liquid interface. &lt;i&gt;IL-6&lt;\/i&gt; and &lt;i&gt;IL-8&lt;\/i&gt; mRNA expression was examined by real-time PCR, and IL-6 and IL-8 secretion was measured by ELISA after stimulation of HCEC with histamine. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Histamine upregulated &lt;i&gt;IL-6&lt;\/i&gt; and &lt;i&gt;IL-8&lt;\/i&gt; expression and IL-6 and IL-8 secretion in a dose-dependent manner in HCEC. Niflumic acid (NFA), an hCLCA blocker, reduced histamine-induced IL-6 and IL-8 expression. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Histamine-induced IL-6 and IL-8 production could be attenuated by NFA, an hCLCA blocker.<\/jats:p>","DOI":"10.1159\/000354177","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T11:55:26Z","timestamp":1378900526000},"page":"192-196","source":"Crossref","is-referenced-by-count":4,"title":["Niflumic Acid Reduces Histamine-Induced Interleukin-6 and -8 Expression in Human Conjunctival Epithelial Cells"],"prefix":"10.1159","volume":"50","author":[{"given":"Sung A.","family":"Lim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu Yeon","family":"Hwang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So-Hyang","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,9,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(90)90075-F"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(94)70072-9"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.111238"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0105"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.117.5.643"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-006-0353-z"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1172\/JCI22433"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200110000-00014"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/1043-4666(92)90028-P"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159\/000325026"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.04.005"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06-2031"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2004.055046"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(94)90148-1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.10.4885"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/354177","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/354177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:42:42Z","timestamp":1745433762000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000354177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":15,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2013,11,1]]},"published-print":{"date-parts":[[2013,11,4]]}},"URL":"https:\/\/doi.org\/10.1159\/000354177","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5241547","asserted-by":"subject"}]},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"type":"print","value":"0030-3747"},{"type":"electronic","value":"1423-0259"}],"subject":[],"published":{"date-parts":[[2013]]}}}