{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T18:21:54Z","timestamp":1789410114819,"version":"build-2803163510"},"reference-count":23,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2013,12,18]],"date-time":"2013-12-18T00:00:00Z","timestamp":1387324800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,12,18]],"date-time":"2013-12-18T00:00:00Z","timestamp":1387324800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; To investigate if galectin-3: (1) enhances adhesion of rat corneal epithelial cells onto a collagen IV substrate and (2) promotes wound healing in rat corneal explants. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Primary cultures of rat corneal epithelial cells were fixed and immunostained with galectin-3 antibody. To test cellular adherence onto plates coated with collagen type IV, isolated corneal epithelial cells from rats were cultured for 24 h with or without recombinant galectin-3. The attached cells were counted after fixing and staining with 0.1% crystal violet. Direct binding of galectin-3 to collagen IV was tested using a biotin label transfer method. To evaluate wound healing, explants with a 3.5-mm diameter wound in the central corneal epithelium from rats were incubated for 16 h with or without recombinant galectin-3. Changes in the size of the wound were measured with a digital microscope after staining with 5% fluorescein sodium. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; In rat corneal epithelial cells, galectin-3 was stained throughout the cytoplasm, with increasing density adjacent to the plasma membrane. Exogenous galectin-3, but not epidermal growth factor (EGF), significantly promoted adhesion of corneal epithelial cells onto the collagen IV substrate. Galectin-3 directly bound to collagen IV in vitro. Exogenous galectin-3 significantly enhances wound healing in the corneal explants, which was partially inhibited by \u03b2-lactose. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Galectin-3 promotes adhesion of corneal epithelial cells onto collagen IV and enhances wound healing in corneal explants. Since galectin-3 functions in promoting wound healing by a different mechanism than that used by EGF, exogenous galectin-3 may be a candidate drug for enhancing epithelial cell wound healing in disorders of the cornea.<\/jats:p>","DOI":"10.1159\/000355846","type":"journal-article","created":{"date-parts":[[2013,12,18]],"date-time":"2013-12-18T08:34:49Z","timestamp":1387355689000},"page":"96-103","source":"Crossref","is-referenced-by-count":26,"title":["Galectin-3 Enhances Epithelial Cell Adhesion and Wound Healing in Rat Cornea"],"prefix":"10.1159","volume":"51","author":[{"given":"C.","family":"Yabuta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Yano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.R.","family":"Shearer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Azuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2013,12,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007133430202"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(01)00007-6"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(02)00042-3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0642"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.85.11.1336"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200981200"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.045674"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.28.1.37.23493"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2004.11.011"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0266"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2010.956920"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-4573"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.312520"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0302"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0704"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2009.00726.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2006.01.010"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20564"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1021\/bi972958l"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.12.020"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-4380"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404966101"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.266692"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/355846","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/355846","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/355846","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:13:12Z","timestamp":1745431992000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000355846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,18]]},"references-count":23,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,1,1]]},"published-print":{"date-parts":[[2014,1,13]]}},"URL":"https:\/\/doi.org\/10.1159\/000355846","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"value":"0030-3747","type":"print"},{"value":"1423-0259","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,18]]}}}