{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T04:24:36Z","timestamp":1768537476800,"version":"3.49.0"},"reference-count":22,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2013,11,16]],"date-time":"2013-11-16T00:00:00Z","timestamp":1384560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2013,11,16]],"date-time":"2013-11-16T00:00:00Z","timestamp":1384560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; Evaluation of combined morphometry and immunoexpression of cytokeratin 13 (CK13) and cytokeratin 17 (CK17) for cytological identification of superficial oral squamous cells. &lt;b&gt;&lt;i&gt;Study Design:&lt;\/i&gt;&lt;\/b&gt; Smears from 11 tongue squamous cell carcinoma patients were processed by liquid-based cytology, stained via the Papanicolaou method and divided into multiple specimens by cell transfer. Morphometric indices, including nuclear area, nuclear perimeter, nuclear circular rate, largest-to-smallest dimension ratio of the nucleus and nucleocytoplasmic ratio, were measured using a computerized analysis system. CK13 and CK17 were detected by immunostaining. Morphometric values were compared between cell populations with distinct staining and immunoexpression patterns. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Most orange G-stained superficial cells were negative for CK13 (99.4%) and CK17 (98.6%). For light green-stained superficial cells, loss of CK13 was associated with greater cellular atypia in the nuclear area, nuclear perimeter and nucleocytoplasmic ratio (p &lt; 0.01), while expression of CK17 was related to higher-grade cellular atypia in the same parameters (p &lt; 0.01) as well as the nuclear circular rate (p &lt; 0.05). &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Immunoexpression of CK13 and CK17 in light green-stained superficial cells was associated with more severe morphological atypia. Combined morphometry and immunoexpression of CK13 and CK17 might be useful for cytological diagnosis of this cell population.<\/jats:p>","DOI":"10.1159\/000355865","type":"journal-article","created":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T13:57:52Z","timestamp":1384523872000},"page":"67-75","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of Superficial Oral Squamous Cell Malignancy Based on Morphometry and Immunoexpression of Cytokeratin 13 and Cytokeratin 17"],"prefix":"10.1159","volume":"58","author":[{"given":"Mitsumasa","family":"Yamashina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumichi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morio","family":"Tonogi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gen-yuki","family":"Yamane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Katakura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2013,11,16]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345510300030201"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2006.00402.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1971.3"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.1993.tb00070.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2005.tb00346.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2004.02.004"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/BF01606063"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(00)00094-4"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2009.11.012"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.218"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-007-0308-8"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2008.01501.x"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.01.011"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajoms.2010.12.005"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-012-1202-6"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdp.2003.09.001"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2004.01.021"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1995.tb00619.x"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/nature04659"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1038\/ng.665"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2007.11.042"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21063"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/355865","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/355865","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:13:17Z","timestamp":1745431997000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000355865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,16]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,2,1]]},"published-print":{"date-parts":[[2014,1,22]]}},"URL":"https:\/\/doi.org\/10.1159\/000355865","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,16]]}}}