{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:00:55Z","timestamp":1760061655158,"version":"3.40.4"},"reference-count":26,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncology"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; Individuals with decreased thiopurine methyltransferase (TPMT) activity are at risk of adverse effects of thiopurine administration whereas its increased activity may inactivate drugs faster. We evaluated genotype-phenotype correlations in patients with suspected hematological malignancies and inflammatory bowel disease from our region based on findings of nonlinear TPMT enzyme kinetics previously unreported. &lt;b&gt;&lt;i&gt;Patients and Methods:&lt;\/i&gt;&lt;\/b&gt; The study group comprised 267 individuals. They were screened for the most common variants of low TPMT activity. TPMT activity was measured in erythrocytes using the HPLC rate-blanked method. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Thirty-three patients (12.4%) were heterozygous (26 were TPMT*1\/*3A, 5 TPMT*1\/*2, 2 TPMT *1\/*3C) and 1 was a compound heterozygote (*2\/*3A). Normal and low normal TPMT activities substantially overlapped in wild-type and heterozygous individuals, whereas high activities were found in 29 wild-type genotyped patients. Extreme and life-threatening toxicity was observed in the compound heterozygote patient. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Activity measurement performed at diagnosis provides clinicians with information on immediate pharmacokinetic-related adverse events and\/or hypermetabolism, and genotyping may indicate the rate of pharmacodynamic thioguanine nucleotide accumulation due to slower overall thiopurine metabolism.<\/jats:p>","DOI":"10.1159\/000357407","type":"journal-article","created":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T07:51:39Z","timestamp":1394610699000},"page":"152-158","source":"Crossref","is-referenced-by-count":6,"title":["Augmenting Clinical Interpretability of Thiopurine Methyltransferase Laboratory Evaluation"],"prefix":"10.1159","volume":"86","author":[{"given":"Regina","family":"Demlova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Mrkvicova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav","family":"Sterba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Bernatikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Stary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Sukova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Mikuskova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alica","family":"Chocholova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Mladosievicova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Soltysova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darina","family":"Behulova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Pilatova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenka","family":"Zdrazilova-Dubska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalibor","family":"Valik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,3,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(78)90311-X"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1996.15.17"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0b013e32835f1cc0"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200404000-00018"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3508"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2011.03.022"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.04.039"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.155"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24354"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2012.02.017"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1080\/15257770.2013.776685"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.255"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.2165\/00019053-200624080-00004"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1258\/000456306778520070"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-004-0728-1"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.213"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.2174\/157488406784111627"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1258\/0004563041201455"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2002.01353.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199608000-00003"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2011.11.014"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.12066"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2010.320"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2013.4"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.22936"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2010.03867.x"}],"container-title":["Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/357407","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/357407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:27:47Z","timestamp":1745432867000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000357407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,5,1]]},"published-print":{"date-parts":[[2014,5,9]]}},"URL":"https:\/\/doi.org\/10.1159\/000357407","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5125987","asserted-by":"subject"}]},"ISSN":["0030-2414","1423-0232"],"issn-type":[{"type":"print","value":"0030-2414"},{"type":"electronic","value":"1423-0232"}],"subject":[],"published":{"date-parts":[[2014]]}}}