{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:14:21Z","timestamp":1761059661875,"version":"3.40.4"},"reference-count":31,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; To promote understanding of immunoglobulin A nephropathy (IgAN) pathophysiology, we tried to elucidate glomerular protein profiles in IgAN, using microsieving that we established recently to isolate glomeruli from renal biopsy samples and proteomic approaches. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Glomeruli were isolated from renal biopsy samples of patients with IgAN (n = 5) and with minimal change nephrotic syndrome (MCNS; n = 5) using microsieving. Proteins extracted from the isolated glomeruli were separated by 2-dimensional differential gel electrophoresis (2D-DIGE). Proteins with different amounts between the two groups were identified by mass spectrometry. One of the identified proteins, \u03b1-actinin-4 (ACTN4), was further analyzed by Western blotting, RT-polymerase chain reaction (PCR), and immunohistochemistry. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; By 2D-DIGE, 72 out of the detected 1,170 protein spots showed significantly different intensity between the two groups (p &lt; 0.05). Thirty-four out of the 72 protein spots showed more than 1.5-fold or less than 1\/1.5-fold intensity, out of which 16 protein spots were successfully identified. No microbial protein was identified. ACTN4 molecules with a low molecular weight of approximately 77 kDa were found to increase in the IgAN group. Lack of an N-terminal part of ACTN4 was demonstrated by Western blotting. No defect of mRNA for ACTN4 was evidenced by RT-PCR. Predominant existence of ACTN4 in capillary walls of glomeruli of IgAN patients was demonstrated by immunohistochemistry in glomerular sections of patients with IgAN. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Use of microsieving enabled us to biochemically analyze glomerular proteins in renal biopsy samples from patients with glomerular diseases. With this method, we demonstrated skewed glomerular protein profiles in IgAN.<\/jats:p>","DOI":"10.1159\/000357788","type":"journal-article","created":{"date-parts":[[2014,1,15]],"date-time":"2014-01-15T16:34:35Z","timestamp":1389803675000},"page":"36-45","source":"Crossref","is-referenced-by-count":6,"title":["Proteomic Analysis of Whole Glomeruli in Patients with IgA Nephropathy Using Microsieving"],"prefix":"10.1159","volume":"39","author":[{"given":"Shigeki","family":"Kojima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Koitabashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuko","family":"Iizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsumi","family":"Arito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyuki","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manae S.","family":"Kurokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoya","family":"Suematsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yugo","family":"Shibagaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjiro","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,1,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01143.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1159\/000049068"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1159\/000102307"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2007.10.008"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2004.01.002"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002185"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.205"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000320198"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1097\/01.smj.0000243183.87381.c2"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2007.tb03878.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.01.003"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.243"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500739"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.4.417"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)90875-3"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00714.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0169(1999)43:3<186::AID-CM2>3.0.CO;2-1"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.3.627"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2010.12.009"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006010033"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/73456"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.6.1383"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt293"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(02)00255-0"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfg620"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90222.2008"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.03.040"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.00472.x"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2010.010237"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020636308270"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1179\/1973947812Y.0000000049"}],"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/357788","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/357788","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:31:24Z","timestamp":1745433084000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000357788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,2,1]]},"published-print":{"date-parts":[[2014,2,11]]}},"URL":"https:\/\/doi.org\/10.1159\/000357788","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5126068","asserted-by":"subject"}]},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"type":"print","value":"0250-8095"},{"type":"electronic","value":"1421-9670"}],"subject":[],"published":{"date-parts":[[2014]]}}}