{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T10:56:44Z","timestamp":1759229804701,"version":"3.40.4"},"reference-count":30,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Aims:&lt;\/i&gt;&lt;\/b&gt; To observe the effect of TC14012 (a CXCR4 antagonist and CXCR7 agonist) on alkali burn-induced corneal neovascularization (CNV) in a mouse model. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; CNV was induced in vivo by alkali burns on the corneas of BALB\/c mice. A total of 54 mice treated with alkali burns were randomly divided into 3 groups, each of which received one of the following treatments: bilateral subconjunctival injections of TC14012 for 3 consecutive days, bilateral subconjunctival injections of balanced saline (BS) for 3 consecutive days or no treatment (blank control). The areas of CNV were measured on days 3, 7 and 14 after the alkali burns. CXCR4, CXCR7, vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP) mRNAs were detected and quantified by real-time reverse transcription PCR on days 7 and 14. Additionally, the expression of the proteins CXCR4, CXCR7, VEGF, \u03b2-arrestin 2, total ERK1\/2 and phospho-ERK1\/2 was determined by Western blotting. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; On day 7 after the alkali burns, the CNV area, VEGF, MMP-2 and MMP-9 mRNA levels, and VEGF, \u03b2-arrestin 2 and phospho-ERK1\/2 protein levels were increased in the TC14012 group compared with the nontreatment and BS groups. However, on day 14, the CNV area, CXCR4, CXCR7, VEGF, MMP-2 and MMP-9 mRNA levels, and the CXCR4, CXCR7, VEGF and \u03b2-arrestin 2 protein levels were significantly decreased in the TC14012 group. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; TC14012 initially enhanced alkali burn-induced CNV but reduced CNV in later stages. In addition to CXCR4, CXCR7 is involved in the pathogenesis of CNV.<\/jats:p>","DOI":"10.1159\/000358201","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T06:44:24Z","timestamp":1400049864000},"page":"17-24","source":"Crossref","is-referenced-by-count":8,"title":["The Effect of TC14012 on Alkali Burn-Induced Corneal Neovascularization in Mice"],"prefix":"10.1159","volume":"52","author":[{"given":"Minqian","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanzhi","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,5,14]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097\/00055735-200108000-00002"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(02)00420-9"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2005.04.007"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.04.071"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508234200"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052144"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912852107"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1186\/bcr3074"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610444104"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.3980\/j.issn.2222-3959.2011.01.10"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C110.147470"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1124\/mol.108.053389"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(01)00323-7"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.0412-4081.2012.04.012"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00044911"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20387"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707465200"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042814"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12024"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009175"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.277038"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-68"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043665"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24015"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1002\/art.27650"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034192"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.01.032"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.5045\/kjh.2011.46.4.244"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00010.2007"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.212"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358201","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358201","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:35:32Z","timestamp":1745433332000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000358201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2014,6,1]]},"published-print":{"date-parts":[[2014,6,24]]}},"URL":"https:\/\/doi.org\/10.1159\/000358201","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"type":"print","value":"0030-3747"},{"type":"electronic","value":"1423-0259"}],"subject":[],"published":{"date-parts":[[2014]]}}}