{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:53:27Z","timestamp":1790146407771,"version":"4.0.1"},"reference-count":8,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Objectives: This study investigates potential colposcopy referral rates, as per the latest American Society for Colposcopy and Cervical Pathology recommendations, following the change in high-risk human papillomavirus (HR-HPV) detection methodology from Hybrid Capture 2 (HC2) to APTIMA at our institution. Study Design: Rates of colposcopy referral were compared between two cohorts, each comprising all Pap samples with a diagnosis of atypical squamous cells of undetermined significance (ASCUS) tested for HR-HPV in our laboratory during a 12-month period. Cohorts I and II included Pap samples tested with HC2 (n = 1,856) and APTIMA (n = 1,651), respectively. The rates of quantity not sufficient (QNS) results were determined for all Pap samples during the same time periods. Results: The proportion of HR-HPV-positive Pap samples with an ASCUS diagnosis was significantly lower with APTIMA (42%) than with HC2 (53%; p &lt; 0.0001). APTIMA also resulted in a significantly lower QNS rate among all Pap samples (0.42 vs. 4.3% with HC2; p &lt; 0.0001). Conclusion: The change in HR-HPV detection methodology from HC2 to APTIMA has led to a 21% reduction in colposcopy referrals and a 90% decrease in QNS rates at our institution. The new methodology has resulted in more cost-effective patient care and fewer insufficient samples requiring repeat HR-HPV testing.<\/jats:p>","DOI":"10.1159\/000358246","type":"journal-article","created":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T10:45:07Z","timestamp":1392288307000},"page":"162-166","source":"Crossref","is-referenced-by-count":22,"title":["Testing of Integrated Human Papillomavirus mRNA Decreases Colposcopy Referrals: Could a Change in Human Papillomavirus Detection Methodology Lead to More Cost-Effective Patient Care?"],"prefix":"10.1159","volume":"58","author":[{"given":"Jennifer L.","family":"Sauter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Mount","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy L.","family":"St. John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Wojewoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald J.","family":"Bryant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladwyn","family":"Leiman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2014,2,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1490"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.325.7364.572"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.12.008"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11530"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1042\/CS20050369"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25726"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(09)70009-5"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27636"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/acy\/article-pdf\/58\/2\/162\/3894024\/000358246.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/acy\/article-pdf\/58\/2\/162\/3894024\/000358246.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:35:59Z","timestamp":1745433359000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000358246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":8,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,4,1]]},"published-print":{"date-parts":[[2014,3,21]]}},"URL":"https:\/\/doi.org\/10.1159\/000358246","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}