{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T14:58:26Z","timestamp":1758121106657,"version":"3.40.4"},"reference-count":22,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2014,5,22]],"date-time":"2014-05-22T00:00:00Z","timestamp":1400716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,5,22]],"date-time":"2014-05-22T00:00:00Z","timestamp":1400716800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron Exp Nephrol"],"abstract":"<b><i>Background\/Aims:<\/i><\/b> Continuous exposure of the peritoneal membrane to high-glucose (HG) peritoneal dialysis fluids (PDFs) can produce peritoneal mesothelial cells (PMCs) injury. It has been demonstrated that hydrogen sulfide (H<sub>2<\/sub>S), the third endogenous gaseous mediator identified after nitric oxide and carbon monoxide, exhibits a potent protective effect on cell activity. We studied the toxic effects of HG PDFs and their reversal by H<sub>2<\/sub>S on cultures of rat PMCs. <b><i>Methods:<\/i><\/b> Synchronized confluent rat PMCs were incubated with 2.5% glucose PDFs with or without NaHS, an H<sub>2<\/sub>S donor. Cell viability was assessed by methyl thiazolyl tetrazolium assay and flow cytometry. The level of phospho-p38 mitogen-activated protein kinase (MAPK) was analyzed by immunoblotting. p53, Bax and Bcl-2 mRNA expressions by rat PMCs were detected by real-time PCR. The levels of reactive oxygen species (ROS), superoxide dismutase (SOD) activity and caspase-3 activity were measured. <b><i>Results:<\/i><\/b> Exposure of rat PMCs to 2.5% glucose PDFs for 24 h resulted in a significant induction of apoptosis, which was attenuated by NaHS. NaHS also restored the 2.5% glucose PDF-induced increase in phospho-p38 MAPK (indices of cellular toxicity). Further investigation of the apoptotic mechanisms in rat PMCs demonstrated that HG activated caspase-3 and upregulated Bax, while it downregulated Bcl-2. All the above responses were prevented by pretreatment with NaHS. Moreover, NaHS reversed the 2.5% glucose PDF-induced increase in ROS generation and decrease in SOD activity. <b><i>Conclusions:<\/i><\/b> These findings suggest that HG PDFs significantly inhibit rat PMC viability, leading to peritoneal injury. H<sub>2<\/sub>S exhibits a potent anti-apoptotic ability by attenuating oxidative stress and inhibiting caspase-3 activation, which in turn restores peritoneal injury.<\/jats:p>","DOI":"10.1159\/000358436","type":"journal-article","created":{"date-parts":[[2014,5,22]],"date-time":"2014-05-22T06:56:29Z","timestamp":1400741789000},"page":"157-165","source":"Crossref","is-referenced-by-count":7,"title":["Hydrogen Sulfide Ameliorates High-Glucose Toxicity in Rat Peritoneal Mesothelial Cells by Attenuating Oxidative Stress"],"prefix":"10.1159","volume":"126","author":[{"given":"Ying","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaying","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaosong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongbing","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,5,22]]},"reference":[{"doi-asserted-by":"publisher","key":"ref1","DOI":"10.1046%2Fj.1523-1755.1999.00264.x"},{"doi-asserted-by":"publisher","key":"ref2","DOI":"10.1111%2Fj.1523-1755.2004.00491.x"},{"doi-asserted-by":"publisher","key":"ref3","DOI":"10.1254%2Fjphs.FP0070258"},{"doi-asserted-by":"publisher","key":"ref4","DOI":"10.1016%2Fj.neures.2010.07.2039"},{"doi-asserted-by":"publisher","key":"ref5","DOI":"10.1016%2Fj.neuroscience.2010.02.006"},{"doi-asserted-by":"publisher","key":"ref6","DOI":"10.1038%2Fsj.bjp.0706993"},{"doi-asserted-by":"publisher","key":"ref7","DOI":"10.1016%2Fj.freeradbiomed.2004.10.015"},{"doi-asserted-by":"publisher","key":"ref8","DOI":"10.2337%2Fdiabetes.50.6.1472"},{"doi-asserted-by":"publisher","key":"ref9","DOI":"10.1159%2F000223778"},{"doi-asserted-by":"publisher","key":"ref10","DOI":"10.3747%2Fpdi.2010.00164"},{"doi-asserted-by":"publisher","key":"ref11","DOI":"10.1046%2Fj.1523-1755.2003.00836.x"},{"doi-asserted-by":"publisher","key":"ref12","DOI":"10.1186%2Fcc7700"},{"doi-asserted-by":"publisher","key":"ref13","DOI":"10.1111%2Fj.1476-5381.2010.01119.x"},{"doi-asserted-by":"publisher","key":"ref14","DOI":"10.1097%2FFJC.0b013e31823b4915"},{"doi-asserted-by":"publisher","key":"ref15","DOI":"10.1093%2Fintimm%2Fdxh229"},{"doi-asserted-by":"publisher","key":"ref16","DOI":"10.2215%2FCJN.05720809"},{"doi-asserted-by":"publisher","key":"ref17","DOI":"10.1126%2Fscience.1104343"},{"doi-asserted-by":"publisher","key":"ref18","DOI":"10.1371%2Fjournal.pone.0036268"},{"doi-asserted-by":"publisher","key":"ref19","DOI":"10.1016%2Fj.freeradbiomed.2008.09.014"},{"doi-asserted-by":"publisher","key":"ref20","DOI":"10.1152%2Fajprenal.00145.2009"},{"doi-asserted-by":"publisher","key":"ref21","DOI":"10.1097%2FSHK.0b013e318180ff89"},{"doi-asserted-by":"publisher","key":"ref22","DOI":"10.1161%2Fhh1201.092094"}],"container-title":["Nephron Experimental Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358436","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358436","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T18:37:16Z","timestamp":1745433436000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000358436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,22]]},"references-count":22,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,6,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000358436","archive":["Portico"],"relation":{},"ISSN":["1660-2129"],"issn-type":[{"type":"electronic","value":"1660-2129"}],"subject":[],"published":{"date-parts":[[2014,5,22]]}}}