{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:01:57Z","timestamp":1771261317394,"version":"3.50.1"},"reference-count":14,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; To evaluate the use of a new immunocytochemical dual-staining protocol, which allows for the detection of coexpression of the p16&lt;sup&gt;INK4a&lt;\/sup&gt; (p16) and Ki-67 biomarkers in prenatal care. It was hypothesized that dual-staining cytology may predict postpartum outcome in patients with abnormal cervical Papanicolaou (Pap) smears during pregnancy. &lt;b&gt;&lt;i&gt;Study Design:&lt;\/i&gt;&lt;\/b&gt; In this retrospective pilot study, 27 abnormal Pap smears collected from women during early pregnancy were destained and subsequently stained for p16\/Ki-67. Results were correlated with histologic outcome collected during postpartum follow-up. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Fourteen of 20 abnormal Pap smears during pregnancy showed a positive p16\/Ki-67 dual-stained cytology result, whereas 6 specimens tested negative. Seven cases were excluded due to technical reasons. All 14 patients who were positive for p16\/Ki-67 dual stain had cervical intraepithelial neoplasia (CIN) on postpartum histology. In contrast, 5 out of 6 patients negative for p16\/Ki-67 dual-staining had a negative histology postpartum and during follow-up, and the remaining patient showed a CIN1 (p &lt; 0.001). &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; p16\/Ki-67 dual-stained cytology may provide a valuable novel approach to identify pregnant women with persistent or progressing CIN disease and may help improve the management of abnormal Pap cytology results during pregnancy.<\/jats:p>","DOI":"10.1159\/000358817","type":"journal-article","created":{"date-parts":[[2014,3,7]],"date-time":"2014-03-07T07:46:46Z","timestamp":1394178406000},"page":"293-296","source":"Crossref","is-referenced-by-count":4,"title":["p16\/Ki-67 Dual-Stained Cytology Testing May Predict Postpartum Outcome in Patients with Abnormal Papanicolaou Cytology during Pregnancy"],"prefix":"10.1159","volume":"58","author":[{"given":"Gerda","family":"Trutnovsky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassiliki","family":"Kolovetsiou-Kreiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf","family":"Reich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,3,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0412.2000.079004306.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.01.043"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1997.4856"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2010.04.016"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2008.00636.x"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/01.grf.0000151570.53696.04"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0412.2011.01092.x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.02.033"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000331047"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20188"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-0270"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2012.05.004"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.06.011"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20140"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358817","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/358817","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:25:40Z","timestamp":1745429140000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000358817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":14,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,6,1]]},"published-print":{"date-parts":[[2014,7,3]]}},"URL":"https:\/\/doi.org\/10.1159\/000358817","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}