{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:54:12Z","timestamp":1777442052883,"version":"3.51.4"},"reference-count":31,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Surg Res"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Ischemia-reperfusion (I\/R) models have shown that C-reactive protein (CRP) and immunoglobulin M (IgM) are involved in complement activation. Binding of CRP and IgM to damaged cell membranes initiates complement activation and aggravates I\/R injury in various organs. However, the time course of CRP- and IgM-mediated complement activation and the relation to hepatocellular injury and inflammation in liver I\/R are unknown. &lt;b&gt;&lt;i&gt;Aim:&lt;\/i&gt;&lt;\/b&gt; To evaluate the time course of IgM- and CRP-related complement activation and the relation to hepatocellular injury and inflammation in a hepatic I\/R rat model. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Male Wistar rats were allocated to (1) five groups of animals exposed to 60 min of partial ischemia (70%) induced via clamping of the left segmental portal triad, followed by 0, 3, 6, 12 or 24 h of reperfusion (n = 6 in each group); (2) five groups of sham-operated animals with corresponding reperfusion times (n = 5), and (3) a control group sacrificed before ischemia (n = 5). Hepatocellular injury, inflammatory response, rat plasma CRP and IgM levels and immunohistochemical depositions of CRP, IgM and C3 were assessed for each group. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Histopathological injury scores of hematoxylin and eosin sections of ischemic liver lobes demonstrated increasing values throughout the reperfusion time with a peak at 12 h. Plasma aminotransferases (alanine aminotransferase and aspartate aminotransferase) significantly increased after 3 h of reperfusion, peaking at 6 h (3,100 \u00b1 800 U\/l; p &lt; 0.05). Hepatic neutrophil influx significantly increased from 3 to 6 h of reperfusion (p &lt; 0.05) and demonstrated the highest value at 12 h (1.1 \u00b1 0.2 U\/mg of protein). Plasma IL-6 levels in the ischemia groups showed peak values after 6 h of reperfusion, decreasing significantly thereafter (p &lt; 0.05). Plasma CRP values reached highest levels after 3 h of reperfusion (mean 91 \u00b1 5% of control pool), decreasing significantly thereafter. Rat IgM concentrations in plasma did not significantly change throughout the reperfusion time. Immunohistochemical depositions of IgM, CRP and C3 in ischemic lobes demonstrated a similar pattern in time, reaching maximum values at 12 h of reperfusion. The percentages of depositions of CRP and IgM were significantly correlated [r(S) = 0.569; p &lt; 0.001; Spearman test]. The time course of C3 and CRP depositions throughout reperfusion and C3 and IgM staining were significantly similar [r(S) = 0.797 and r(S) = 0.656, respectively; p &lt; 0.0001; ANOVA]. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; CRP and IgM depositions demonstrate a parallel time course throughout the reperfusion to hepatocellular damage, inflammatory response and activated complement deposition in this rat hepatic I\/R model. Furthermore, the time course of CRP and IgM depositions was significantly similar to that of activated complement depositions.<\/jats:p>","DOI":"10.1159\/000360474","type":"journal-article","created":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T07:51:39Z","timestamp":1394610699000},"page":"50-62","source":"Crossref","is-referenced-by-count":7,"title":["Immunoglobulin M, C-Reactive Protein and Complement Activation in Rat Hepatic Ischemia-Reperfusion Injury"],"prefix":"10.1159","volume":"52","author":[{"given":"Gwendolyn M.P.","family":"Diepenhorst","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilmar","family":"de Graaf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans W.","family":"Niessen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arl\u00e8ne K.","family":"van Vliet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Erik","family":"Hack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas M.","family":"van Gulik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,3,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007%2Fs00534-002-0730-x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1053%2Fgast.1997.v112.pm9041253"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002%2Flt.22117"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.transproceed.2005.03.134"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.jss.2006.09.018"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1146%2Fannurev.immunol.20.083001.084359"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038%2Fnm0602-582"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1097%2F00024382-200405000-00002"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3389%2Ffimmu.2012.00341"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.surg.2004.05.016"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1073%2Fpnas.0400347101"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.surg.2005.05.028"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1093%2Fcvr%2Fcvq141"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.molimm.2008.06.013"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.imbio.2006.06.011"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1152%2Fajpheart.00049.2009"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1136%2Fjcp.2004.022988"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016%2Fj.surg.2007.05.015"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1084%2Fjem.190.12.1733"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1172%2FJCI200318921"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038%2Fnature04672"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097%2F00024382-200405000-00002"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1432-2277.2000.tb02101.x"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111%2Fj.1365-2249.2005.02958.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016%2FS0168-8278%2800%2980247-0"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1002%2Fbjs.5275"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1046%2Fj.1365-2567.2003.01681.x"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016%2F0161-5890%2878%2990052-4"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1002%2Fhep.21060"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016%2F0925-4439%2894%2990109-0"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016%2F0147-9571%2890%2990277-Z"}],"container-title":["European Surgical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/360474","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/360474","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/360474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:30:13Z","timestamp":1745429413000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000360474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":31,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2014,5,1]]},"published-print":{"date-parts":[[2014,5,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000360474","archive":["Portico"],"relation":{},"ISSN":["0014-312X","1421-9921"],"issn-type":[{"value":"0014-312X","type":"print"},{"value":"1421-9921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}