{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:14:24Z","timestamp":1787933664958,"version":"build-2784847793"},"reference-count":23,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Recombinant human erythropoietin (rh-EPO) has been used as a drug to treat premature infant anemia for over a decade. In addition to its erythropoietic effect, rh-EPO has also been reported to have protective effects against brain injury. &lt;b&gt;&lt;i&gt;Objectives:&lt;\/i&gt;&lt;\/b&gt; Our aim was to evaluate the levels of angiogenesis-related cells (CD34+ cells) and angiogenic factors (vascular endothelial growth factor, VEGF, and angiopoietin-1, Ang-1) in a neonatal rat model of cerebral unilateral hypoxia-ischemia (HI) and to identify the effects of rh-EPO on angiogenic responses. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Postnatal day 3 (PD3) rats underwent permanent ligation of the right common carotid artery followed by 6% O&lt;sub&gt;2&lt;\/sub&gt; for 4 h (HI) or sham operation and normoxic exposure (sham). Immediately after HI, the rats received a single intraperitoneal injection of rh-EPO (5 U\/g) or saline. Angiogenesis-related cells (CD34+ cells) and angiogenic factors (VEGF and Ang-1) were examined on PD5, 7, 10 and 14. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Compared with the sham rats, the number of CD34+ cells in HI rats increased from PD5 to 7 but decreased from PD10 to 14. VEGF and Ang-1 mRNA levels both increased from PD5 to 14. CD34+ cells, VEGF and Ang-1 were all upregulated in rh-EPO-treated rats compared with HI rats. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; In the present study, we show the angiogenic effects of rh-EPO in a rat model of neonatal cerebral unilateral HI. Our results highlight the powerful therapeutic potential of rh-EPO treatment of HI premature brain for the enhancement of angiogenic responses.<\/jats:p>","DOI":"10.1159\/000362262","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T06:41:34Z","timestamp":1403592094000},"page":"143-148","source":"Crossref","is-referenced-by-count":16,"title":["Recombinant Human Erythropoietin Augments Angiogenic Responses in a Neonatal Rat Model of Cerebral Unilateral Hypoxia-Ischemia"],"prefix":"10.1159","volume":"106","author":[{"given":"Lihua","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2014,6,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1008-8830.2005.01.006"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(08)70294-1"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1515\/jpme.1995.23.1-2.127"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000341615"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-007-9073-3"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0b013e3181d27138"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3171\/2010.5.PEDS1032"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-2701"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2008-0733"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-013-1076-6"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2011.10.058"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159\/000096969"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.2337\/dc07-1125"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21919"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehm177"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2011.01.099"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2009.10.017"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/74725"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22702"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2014.8728"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.surneu.2008.02.040"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e328336eb57"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2008.02024.x"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:37:18Z","timestamp":1745429838000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000362262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":23,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,8,1]]},"published-print":{"date-parts":[[2014,8,7]]}},"URL":"https:\/\/doi.org\/10.1159\/000362262","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"value":"1661-7800","type":"print"},{"value":"1661-7819","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}