{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:31:17Z","timestamp":1745469077583,"version":"3.40.4"},"reference-count":9,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Proteinase-activated receptor 2 (PAR2) may be implicated in skin disorders. Intracellular calcium mobilization is a key step in PAR2-induced signaling. &lt;b&gt;&lt;i&gt;Aims:&lt;\/i&gt;&lt;\/b&gt; In this study, we investigated the effects of nifedipine, verapamil, and low-molecular-weight heparin (LMWH) on SLIGRL-NH&lt;sub&gt;2&lt;\/sub&gt;-induced PAR2-mediated calcium mobilization within cells. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; The intracellular calcium concentration was measured with fluo-8, a fluorescence indicator for free Ca&lt;sup&gt;2+&lt;\/sup&gt;. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Our results showed that SLIGRL-NH&lt;sub&gt;2&lt;\/sub&gt; induced a dose-dependent calcium influx. This calcium influx was completely blocked in HaCaT cells and significantly blocked by LMWH in HEK293\/PAR2 cells. However, both nifedipine and verapamil failed to inhibit the SLIGRL-NH&lt;sub&gt;2&lt;\/sub&gt;-induced calcium influx in either cell line. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; These results indicate that the PAR2 activation-induced calcium mobilization was mediated by intracellular calcium stores but not by extracellular calcium present in the media.<\/jats:p>","DOI":"10.1159\/000362283","type":"journal-article","created":{"date-parts":[[2014,6,11]],"date-time":"2014-06-11T08:19:26Z","timestamp":1402474766000},"page":"216-219","source":"Crossref","is-referenced-by-count":0,"title":["A Comparison of the Effects of Nifedipine, Verapamil, and Low-Molecular-Weight Heparin on SLIGRL-NH&lt;sub&gt;2&lt;\/sub&gt;-Induced Calcium Influx through Proteinase-Activated Receptor 2 Activation"],"prefix":"10.1159","volume":"93","author":[{"given":"Jun","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Dou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2014,6,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.20.9208"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600204"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.17.11133"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-6705.2006.00430.x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.177428"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.36.22003"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9151"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1124\/mol.109.055509"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.201988"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362283","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/362283","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:37:27Z","timestamp":1745429847000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000362283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":9,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2014,9,1]]},"published-print":{"date-parts":[[2014,9,9]]}},"URL":"https:\/\/doi.org\/10.1159\/000362283","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"type":"print","value":"0031-7012"},{"type":"electronic","value":"1423-0313"}],"subject":[],"published":{"date-parts":[[2014]]}}}